Generation of c-MycERTAM-transduced human late-adherent olfactory mucosa cells for potential regenerative applications


Human olfactory mucosa cells (hOMCs) have been transplanted to the damaged spinal cord both pre-clinically and clinically. To date mainly autologous cells have been tested. However, inter-patient variability in cell recovery and quality, and the fact that the neuroprotective olfactory ensheathing cell (OEC) subset is difficult to isolate, means an allogeneic hOMC therapy would be an attractive “off-the-shelf” alternative. The aim of this study was to generate a candidate cell line from late-adherent hOMCs, thought to contain the OEC subset. Primary late-adherent hOMCs were transduced with a c-MycERTAM gene that enables cell proliferation in the presence of 4-hydroxytamoxifen (4-OHT). Two c-MycERTAM-derived polyclonal populations, PA5 and PA7, were generated and expanded. PA5 cells had a normal human karyotype (46, XY) and exhibited faster growth kinetics than PA7, and were therefore selected for further characterisation. PA5 hOMCs express glial markers (p75NTR, S100ß, GFAP and oligodendrocyte marker O4), neuronal markers (nestin and ß-III-tubulin) and fibroblast-associated markers (CD90/Thy1 and fibronectin). Co-culture of PA5 cells with a neuronal cell line (NG108-15) and with primary dorsal root ganglion (DRG) neurons resulted in significant neurite outgrowth after 5 days. Therefore, c-MycERTAM-derived PA5 hOMCs have potential as a regenerative therapy for neural cells.

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Divisions: College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences
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Uncontrolled Keywords: General
Publication ISSN: 2045-2322
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Related URLs: http://www.natu ... 598-019-49315-6 (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2019-09-13
Accepted Date: 2019-07-31
Authors: Santiago-toledo, Gerardo
Georgiou, Melanie
Dos Reis, Joana
Roberton, Victoria H.
Valinhas, Ana
Wood, Rachael C.
Phillips, James B.
Mason, Chris
Li, Daqing
Li, Ying
Sinden, John D.
Choi, David
Jat, Parmjit S.
Wall, Ivan B. (ORCID Profile 0000-0001-6294-8348)



Version: Published Version

License: Creative Commons Attribution

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