Minimal regulation of platelet activity by PECAM-1

Abstract

PECAM-1 is a member of the superfamily of immunoglobulins (Ig) and is expressed on platelets at moderate level. PECAM-1 has been reported to have contrasting effects on platelet activation by the collagen receptor GPVI and the integrin, αIIbβ3, even though both receptors signal through Src-kinase regulation of PLCγ2. The present study compares the role of PECAM-1 on platelet activation by these two receptors and by the lectin receptor, CLEC-2, which also signals via PLCγ2. Studies using PECAM-1 knockout-mice and cross-linking of PECAM-1 using specific antibodies demonstrated a minor inhibitory role on platelet responses to the above three receptors and also under some conditions to the G-protein agonist thrombin. The degree of inhibition was considerably less than that produced by PGI2, which elevates cAMP. There was no significant difference in thrombus formation on collagen in PECAM-1-/- platelets relative to litter-matched controls. The very weak inhibitory effect of PECAM-1 on platelet activation relative to that of PGI2 indicate that the Ig-receptor is not a major regulator of platelet activation. PECAM-1 has been reported to have contrasting effects on platelet activation. The present study demonstrates a very mild or negligible effect on platelet activation in response to stimulation by a variety of agonists, thereby questioning the physiological role of the immunoglobulin receptor as a major regulator of platelet activation.

Publication DOI: https://doi.org/10.1080/09537100600881396
Divisions: College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences
Additional Information: This is an Accepted Manuscript of an article published by Taylor & Francis Group in Platelets on Feb 2007, available online at: http://10.1080/09537100600881396
Uncontrolled Keywords: CLEC-2,GPVI,Integrin αβ,PECAM-1,Platelet activation,Hematology,Cell Biology
Publication ISSN: 1369-1635
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2007-02-01
Authors: Dhanjal, Tarvinder S.
Ross, Ewan A. (ORCID Profile 0000-0001-5733-9361)
Auger, Jocelyn M.
Mccarty, Owen J T
Hughes, Craig E.
Senis, Yotis A.
Watson, Steve P.

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