Ross, Ewan A., Freeman, Sylvie, Zhao, Yan, Dhanjal, Tarvinder S., Ross, Emma J., Lax, Sian, Ahmed, Zubair, Hou, Tie Zheng, Kalia, Neena, Egginton, Stuart, Nash, Gerard, Watson, Steven P., Frampton, Jon and Buckley, Christopher D. (2008). A novel role for PECAM-1 (CD31) in regulating haematopoietic progenitor cell compartmentalization between the peripheral blood and bone marrow. PLoS ONE, 3 (6),
Abstract
Although the expression of PECAM-1 (CD31) on vascular and haematopoietic cells within the bone marrow microenvironment has been recognized for some time, its physiological role within this niche remains unexplored. In this study we show that PECAM-1 influences steady state hematopoietic stem cell (HSC) progenitor number in the peripheral blood but not the bone marrow compartment PECAM-1-/- is required on both progenitors and bone marrow vascular cels in order for efficient transition between the blood and bone marrow to occur. We have identified key roles for PECAM-1 in both the regulation of HSC migration to the chemokine CXCL12, as well as maintaining levels of the matrix degrading enzyme MMP-9 in the bone marrow vascular niche. Using intravital microscopy and adoptive transfer of either wild type (WT) or PECAM-1-/- bone marrow precursors, we demonstrate that the increase in HSC progenitors in the blood is due in part to a reduced ability to migrate from blood to the bone marrow vascular niche. The findings suggest a novel role for a PECAM-1 as a regulator of resting homeostatic progenitor cell numbers in the blood.
Publication DOI: | https://doi.org/10.1371/journal.pone.0002338 |
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Divisions: | College of Health & Life Sciences College of Health & Life Sciences > School of Biosciences |
Additional Information: | © 2008 Ross et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
Uncontrolled Keywords: | General Biochemistry,Genetics and Molecular Biology,General Agricultural and Biological Sciences |
Publication ISSN: | 1932-6203 |
Last Modified: | 31 Oct 2024 08:33 |
Date Deposited: | 05 Sep 2019 15:25 |
Full Text Link: | |
Related URLs: |
http://www.scop ... tnerID=8YFLogxK
(Scopus URL) https://journal ... al.pone.0002338 (Publisher URL) |
PURE Output Type: | Article |
Published Date: | 2008-06-04 |
Authors: |
Ross, Ewan A.
(
0000-0001-5733-9361)
Freeman, Sylvie Zhao, Yan Dhanjal, Tarvinder S. Ross, Emma J. Lax, Sian Ahmed, Zubair Hou, Tie Zheng Kalia, Neena Egginton, Stuart Nash, Gerard Watson, Steven P. Frampton, Jon Buckley, Christopher D. |