The influence of pH and fluid dynamics on the antibacterial efficacy of 45S5 Bioglass

Abstract

In recent years, there has been considerable interest in the potential antibacterial properties that bioactive glasses may possess. However, there have been several conflicting reports on the antibacterial efficacy of 45S5 Bioglass®. Various mechanisms regarding its mode of action have been proposed, such as changes in the environmental pH, increased osmotic pressure, and ‘needle like’ sharp glass debris which could potentially damage prokaryotic cell walls and thus inactivate bacteria. In this current study, a systematic investigation was undertaken on the antibacterial efficacy of 45S5 Bioglass® on Escherichia coli NCTC 10538 and Staphylococcus aureus ATCO 6538 under a range of clinically relevant scenarios including varying Bioglass® concentration, direct and indirect contact between Bioglass® and microorganisms, static and shaking incubation conditions, elevated and neutralised pH environments. The results demonstrated that under elevated pH conditions, Bioglass® particles has no antibacterial effect on S. aureus whilst, a concentration dependent antibacterial effect against E. coli was observed. However, the antibacterial activity ceased when the pH of the media was neutralised. The results of this current study therefore suggest that the mechanism of antibacterial activity of Bioglass® is associated with changes in the environmental pH; an environment that is less likely to occur in vivo due to buffering of the system.

Publication DOI: https://doi.org/10.1088/1748-6041/11/1/015006
Divisions: College of Engineering & Physical Sciences > School of Infrastructure and Sustainable Engineering > Chemical Engineering & Applied Chemistry
College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences > Cell & Tissue Biomedical Research
College of Health & Life Sciences > Chronic and Communicable Conditions
College of Engineering & Physical Sciences > Aston Institute of Materials Research (AIMR)
Additional Information: © IOP
Uncontrolled Keywords: bioglass,45S5,ph,antibacterial,Bioengineering,Biomaterials,Biomedical Engineering
Publication ISSN: 1748-605X
Last Modified: 03 Jun 2024 07:20
Date Deposited: 19 Aug 2019 10:07
Full Text Link: http://iopscien ... 1/1/015006/meta
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2016-02-02
Accepted Date: 2016-01-07
Submitted Date: 2015-10-26
Authors: Begum, Saima
Johnson, Eustace
Worthington, Tony (ORCID Profile 0000-0002-1906-3357)
Martin, Richard A. (ORCID Profile 0000-0002-6013-2334)

Download

[img]

Version: Accepted Version

| Preview

Export / Share Citation


Statistics

Additional statistics for this record