Evidence of lipid degradation during overnight contact lens wear:gas chromatography mass spectrometry as the diagnostic tool


Purpose. We investigated structural differences in the fatty acid profiles of lipids extracted from ex vivo contact lenses by using gas chromatography mass spectrometry (GCMS). Two lens materials (balafilcon A or lotrafilcon A) were worn on a daily or continuous wear schedule for 30 and 7 days. Methods. Lipids from subject-worn lenses were extracted using 1:1 chloroform: methanol and transmethylated using 5% sulfuric acid in methanol. Fatty acid methyl esters (FAMEs) were collected using hexane and water, and analyzed by GCMS (Varian 3800 GC, Saturn 2000 MS). Results. The gas chromatograms of lens extracts that were worn on a continuous wear schedule showed two predominant peaks, C16:0 and C18:0, both of which are saturated fatty acids. This was the case for balafilcon A and lotrafilcon A lenses. However, the gas chromatograms of lens extracts that were worn on a daily wear schedule showed saturated (C16:0, C18:0) and unsaturated (C16:1 and C18:1) fatty acids. Conclusions. Unsaturated fatty acids are degraded during sleep in contact lenses. Degradation occurred independently of lens material or subject-to-subject variability in lipid deposition. The consequences of lipid degradation are the production of oxidative products, which may be linked to contact lens discomfort.

Publication DOI: https://doi.org/10.1167/iovs.13-12881
Divisions: College of Engineering & Physical Sciences > School of Infrastructure and Sustainable Engineering > Chemical Engineering & Applied Chemistry
College of Engineering & Physical Sciences > Aston Institute of Materials Research (AIMR)
Additional Information: Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc Funding: EPSRC for a research studentship
Uncontrolled Keywords: lipid degradation,contact lens discomfort,overnight contact lens wear
Publication ISSN: 1552-5783
Last Modified: 04 Jan 2024 08:44
Date Deposited: 19 Aug 2019 10:06
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2014-03-20
Published Online Date: 2014-02-25
Authors: Panaser, Amandeep
Tighe, Brian J. (ORCID Profile 0000-0001-9601-8501)



Version: Published Version

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