Mass spectrometry-based methods for identifying oxidized proteins in disease:advances and challenges


Many inflammatory diseases have an oxidative aetiology, which leads to oxidative damage to biomolecules, including proteins. It is now increasingly recognized that oxidative post-translational modifications (oxPTMs) of proteins affect cell signalling and behaviour, and can contribute to pathology. Moreover, oxidized proteins have potential as biomarkers for inflammatory diseases. Although many assays for generic protein oxidation and breakdown products of protein oxidation are available, only advanced tandem mass spectrometry approaches have the power to localize specific oxPTMs in identified proteins. While much work has been carried out using untargeted or discovery mass spectrometry approaches, identification of oxPTMs in disease has benefitted from the development of sophisticated targeted or semi-targeted scanning routines, combined with chemical labeling and enrichment approaches. Nevertheless, many potential pitfalls exist which can result in incorrect identifications. This review explains the limitations, advantages and challenges of all of these approaches to detecting oxidatively modified proteins, and provides an update on recent literature in which they have been used to detect and quantify protein oxidation in disease.

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Divisions: College of Health & Life Sciences > Aston Pharmacy School
College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
College of Health & Life Sciences > Chronic and Communicable Conditions
Additional Information: © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license ( Funding: EPSRC (EP/I017887/1).
Uncontrolled Keywords: Cardiovascular disease;,Chlorotyrosine,Inflammation,LC-MS/MS,Multiple reaction monitoring,Neutral loss scanning,Nitrotyrosine,Oxidative post-translational modification,Precursor ion scanning,Protein carbonyls,Medicine(all),Biochemistry,Molecular Biology
Publication ISSN: 2218-273X
Last Modified: 06 May 2024 07:12
Date Deposited: 19 Aug 2019 09:36
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Review article
Published Date: 2015-04-14
Authors: Verrastro, Ivan
Pasha, Sabah
Jensen, Karina Tveen
Pitt, Andrew R. (ORCID Profile 0000-0003-3619-6503)
Spickett, Corinne M. (ORCID Profile 0000-0003-4054-9279)



Version: Published Version

License: Creative Commons Attribution

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