Interfacing low-energy SAW nebulization with liquid chromatography-mass spectrometry for the analysis of biological samples


Soft ionization methods for the introduction of labile biomolecules into a mass spectrometer are of fundamental importance to biomolecular analysis. Previously, electrospray ionization (ESI) and matrix assisted laser desorption-ionization (MALDI) have been the main ionization methods used. Surface acoustic wave nebulization (SAWN) is a new technique that has been demonstrated to deposit less energy into ions upon ion formation and transfer for detection than other methods for sample introduction into a mass spectrometer (MS). Here we report the optimization and use of SAWN as a nebulization technique for the introduction of samples from a low flow of liquid, and the interfacing of SAWN with liquid chromatographic separation (LC) for the analysis of a protein digest. This demonstrates that SAWN can be a viable, low-energy alternative to ESI for the LC-MS analysis of proteomic samples.

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Divisions: College of Health & Life Sciences > Aston Pharmacy School
College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences > Chronic and Communicable Conditions
College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
Additional Information: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission fromthe license holder in order to reproduce thematerial. To view a copy of this license, visit Funding: EPSRC (EP/I017887/1; P/K027611/1); Aston University; ERC advanced investigator award (340117 – Biophonics). Supplementary information accompanies this paper at scientificreports
Uncontrolled Keywords: General
Publication ISSN: 2045-2322
Last Modified: 03 Jun 2024 07:15
Date Deposited: 19 Aug 2019 09:36
Full Text Link: http://www.natu ... /srep09736.html
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2015-05-15
Authors: Tveen Jensen, Karina
Gesellchen, F.
Wilson, Rab
Spickett, Corinne (ORCID Profile 0000-0003-4054-9279)
Cooper, Jonathan M.
Pitt, Andrew (ORCID Profile 0000-0003-3619-6503)



Version: Published Version

License: Creative Commons Attribution

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