Intracellular uptake of EGCG-loaded deformable controlled release liposomes for skin cancer


Caucasian population groups have a higher propensity to develop skin cancer, and associated clinical interventions often present substantial financial burden on healthcare services. Conventional treatments are often not suitable for all patient groups as a result of poor efficacy and toxicity profiles. The primary objective of this study was to develop a deformable liposomal formulation, the properties of which being dictated by the surfactant Tween 20, for the dermal cellular delivery of epigallocatechin gallatein (EGCG), a compound possessing antineoplastic properties. The results demonstrated a significant (p ≤ 0.05) decrease in liposome deformability index (74 ± 8 to 37 ± 7) as Tween 20 loading increased from 0 to 10% w/w, indicating an increase in elasticity. EGCG release over 24-h demonstrated Tween 20 incorporation directly increased release from 13.7% ± 1.1% to 94.4% ± 4.9% (for 0 and 10% w/w Tween 20 respectively). Finally, we demonstrated DilC-loaded deformable liposomes were localized intracellularly within human dermal fibroblast and keratinocyte cells within 2 h. Thus, it was evident that deformable liposomes may aid drug penetration into dermal cells and would be useful in developing a controlled-release formulation.

Publication DOI:
Divisions: College of Health & Life Sciences > Aston Medical School
College of Health & Life Sciences > Aston Pharmacy School
Additional Information: This is an Accepted Manuscript of an article published by Taylor & Francis Group in Journal of Liposome Research on 9 May 2019, available online at:
Uncontrolled Keywords: Skin cancer,Controlled release,Dermal release,Elastic liposomes,Deformable liposomes,Pharmaceutical Science
Publication ISSN: 1532-2394
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://www.tan ... rnalCode=ilpr20 (Publisher URL)
PURE Output Type: Article
Published Date: 2019-05-09
Accepted Date: 2019-03-18
Authors: Marwah, Mandeep (ORCID Profile 0000-0003-4881-003X)
Badhan, Raj K.S. (ORCID Profile 0000-0002-0904-9324)
Lowry, Deborah
Perrie, Yvonne



Version: Accepted Version

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