Enhanced chlorhexidine skin penetration with eucalyptus oil


Background Chlorhexidine digluconate (CHG) is a widely used skin antiseptic, however it poorly penetrates the skin, limiting its efficacy against microorganisms residing beneath the surface layers of skin. The aim of the current study was to improve the delivery of chlorhexidine digluconate (CHG) when used as a skin antiseptic. Method Chlorhexidine was applied to the surface of donor skin and its penetration and retention under different conditions was evaluated. Skin penetration studies were performed on full-thickness donor human skin using a Franz diffusion cell system. Skin was exposed to 2% (w/v) CHG in various concentrations of eucalyptus oil (EO) and 70% (v/v) isopropyl alcohol (IPA). The concentration of CHG (µg/mg of skin) was determined to a skin depth of 1500 µm by high performance liquid chromatography (HPLC). Results The 2% (w/v) CHG penetration into the lower layers of skin was significantly enhanced in the presence of EO. Ten percent (v/v) EO in combination with 2% (w/v) CHG in 70% (v/v) IPA significantly increased the amount of CHG which penetrated into the skin within 2 min. Conclusion The delivery of CHG into the epidermis and dermis can be enhanced by combination with EO, which in turn may improve biocide.

Publication DOI: https://doi.org/10.1186/1471-2334-10-278
Divisions: College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences > Chronic and Communicable Conditions
College of Health & Life Sciences
College of Health & Life Sciences > Aston Pharmacy School
Additional Information: © 2010 Karpanen et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords: 2-propanol,topical administration,local anti-infective agents,chlorhexidine,drug carriers,eucalyptus,humans,monoterpenes,volatile oils,skin,Infectious Diseases
Publication ISSN: 1471-2334
Last Modified: 19 Jun 2024 07:09
Date Deposited: 19 Aug 2019 08:53
Full Text Link: http://www.biom ... 471-2334/10/278
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2010-09-22
Authors: Karpanen, Tarja J
Conway, Barbara R
Worthington, Tony (ORCID Profile 0000-0002-1906-3357)
Hilton, Anthony C (ORCID Profile 0000-0001-8025-5270)
Elliott, Tom S J
Lambert, Peter A (ORCID Profile 0000-0002-8243-2741)



Version: Published Version

License: Creative Commons Attribution

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