Free radical reactions in atherosclerosis; An EPR spectrometry study


The copper catalysed oxidation of homocysteine has been studied by electron paramagnetic resonance (EPR) spectroscopy and spin trapping techniques to determine the nature of free radical species formed under varying experimental conditions. Three radicals; thiyl, alkyl and hydroxyl were detected with hydroxyl being predominant. A reaction mechanism is proposed involving Fenton chemistry. Inclusion of catalase to test for intermediate generation of hydrogen peroxide showed a marked reduction in amount of hydroxyl radical generated. In contrast, the addition of superoxide dismutase showed no significant effect on the level of hydroxyl radical formed. Enhanced radical formation was observed at higher levels of oxygen, an effect which has consequences for differential oxygen levels in arterial and venous systems. Implications are drawn for a higher incidence of atherosclerotic plaque formation in arteries versus veins. © 2006 - IOS Press and the authors. All rights reserved.

Divisions: College of Health & Life Sciences
College of Health & Life Sciences > Chronic and Communicable Conditions
Publication ISSN: 0712-4813
Last Modified: 29 Nov 2023 10:08
Date Deposited: 19 Aug 2019 08:51
Full Text Link: 10.1155/2006/474183
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2006-05-18
Authors: Taiwo, F. A.
Powers, H. J.
Nakano, E.
Griffiths, Helen R.
Nugent, D. F.



Version: Published Version

License: Creative Commons Attribution

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