Role of β3-adrenergic receptors in the action of a tumour lipid mobilizing factor


Induction of lipolysis in murine white adipocytes, and stimulation of adenylate cyclase in adipocyte plasma membranes, by a tumour-produced lipid mobilizing factor, was attenuated by low concentrations (10-7-10-5M) of the specific β3-adrenoceptor antagonist SR59230A. Lipid mobilizing factor (250 nM) produced comparable increases in intracellular cyclic AMP in CHOKI cells transfected with the human β3-adrenoceptor to that obtained with isoprenaline (1 nM). In both cases cyclic AMP production was attenuated by SR59230A confirming that the effect is mediated through a β3-adrenoceptor. A non-linear regression analysis of binding of lipid mobilizing factor to the β3-adrenoceptor showed a high affinity binding site with a Kd value 78±45 nM and a Bmax value (282±1 fmole mg protein-1) comparable with that of other β3-adrenoceptor agonists. These results suggest that lipid mobilizing factor induces lipolysis through binding to a β3-adrenoceptor. © 2002 The Cancer Research Campaign.

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Divisions: College of Health & Life Sciences > Aston Pharmacy School
College of Health & Life Sciences
College of Health & Life Sciences > Chronic and Communicable Conditions
Additional Information: © 2002 The Cancer Research Campaign Creative Commons Attribution-NonCommercial-Share-Alike 3.0 licence, subject to the conditions listed at
Uncontrolled Keywords: β3-adrenoceptor,cachexia,energy metabolism,lipid mobilizing factor,Cancer Research,Oncology
Publication ISSN: 1532-1827
Last Modified: 23 Apr 2024 17:28
Date Deposited: 19 Aug 2019 08:49
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
http://www.natu ... l/6600086a.html (Publisher URL)
PURE Output Type: Article
Published Date: 2002-02
Authors: Russell, Steve T. (ORCID Profile 0000-0002-5491-900X)
Hirai, K.
Tisdale, Michael J.

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