Performance and flow dynamics studies of polymeric optofluidic sers sensors

Abstract

We present a polymer-based optofluidic surface enhanced Raman scattering chip for biomolecule detection, serving as a disposable sensorchoice with cost-effective production. The SERS substrate is fabricated by using industrial roll-to-roll UV-nanoimprinting equipment andintegrated with adhesive-based polymeric microfluidics. The functioning of the SERS detection on-chip is confirmed and the effect of thepolymer lid on the obtainable Raman spectra is analysed. Rhodamine 6G is used as a model analyte to demonstrate continuous flowmeasurements on a planar SERS substrate in a microchannel. The relation between the temporal response of the sensors and sample flowdynamics is studied with varied flow velocities, using SERS and fluorescence detection. The response time of the surface-dependent SERSsignal is longer than the response time of the fluorescence signal of the bulk flow. This observation revealed the effect of convection on thetemporal SERS responses at 25 μl/min to 1000 μl/min flow velocities. The diffusion of analyte molecules from the bulk concentration intothe sensing surface induces about a 40-second lag time in the SERS detection. This lag time, and its rising trend with slower flow velocities, has to be taken into account in future trials of the optofluidic SERS sensor, with active analyte binding on the sensing surface.

Publication DOI: https://doi.org/10.2971/jeos.2015.15043
Divisions: College of Engineering & Physical Sciences > School of Engineering and Technology > Mechanical, Biomedical & Design
College of Engineering & Physical Sciences
Additional Information: The journal is fully open access. This means that anybody is free to read papers published by this journal without the need to register with the journal. On top of the open access, the authors of a published paper have the full copyright of their publication.
Uncontrolled Keywords: Biosensor,Continuous,Flow,Optofluidic,Polymer,SERS,Atomic and Molecular Physics, and Optics
Publication ISSN: 1990-2573
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://www.jeo ... view/15043/1400 (Publisher URL)
PURE Output Type: Article
Published Date: 2015-09-15
Authors: Uusitalo, S.
Hiltunen, J.
Karioja, P.
Siitonen, S.
Kontturi, V.
Myllylä, R.
Kinnunen, M.
Meglinski, I. (ORCID Profile 0000-0002-7613-8191)

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