Optical tweezers study of red blood cell aggregation and disaggregation in plasma and protein solutions

Abstract

Kinetics of optical tweezers (OT)-induced spontaneous aggregation and disaggregation of red blood cells (RBCs) were studied at the level of cell doublets to assess RBC interaction mechanics. Measurements were performed under in vitro conditions in plasma and fibrinogen and fibrinogen + albumin solutions. The RBC spontaneous aggregation kinetics was found to exhibit different behavior depending on the cell environment. In contrast, the RBC disaggregation kinetics was similar in all solutions qualitatively and quantitatively, demonstrating a significant contribution of the studied proteins to the process. The impact of the study on assessing RBC interaction mechanics and the protein contribution to the reversible RBC aggregation process is discussed.

Publication DOI: https://doi.org/10.1117/1.JBO.21.3.035001
Divisions: College of Engineering & Physical Sciences > School of Engineering and Technology > Mechanical, Biomedical & Design
College of Engineering & Physical Sciences
Additional Information: © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. [DOI: 10.1117/1.JBO.21.3.035001]
Uncontrolled Keywords: aggregation,albumin,blood microcirculation,cells interaction,fibrinogen,optical tweezers,red blood cell,Electronic, Optical and Magnetic Materials,Biomaterials,Atomic and Molecular Physics, and Optics,Biomedical Engineering
Publication ISSN: 1560-2281
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://www.spi ... 5001.full?SSO=1 (Publisher URL)
PURE Output Type: Article
Published Date: 2016-03-08
Accepted Date: 2016-02-03
Authors: Lee, Kisung
Kinnunen, Matti
Khokhlova, Maria D.
Lyubin, Evgeny V.
Priezzhev, Alexander V.
Meglinski, Igor (ORCID Profile 0000-0002-7613-8191)
Fedyanin, Andrey A.

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