Parallel in vivo monitoring of pH in gill capillaries and muscles of fishes using microencapsulated biomarkers


Tracking physiological parameters in different organs within the same organism simultaneously and in real time can provide an outstanding representation of the organism's physiological status. The state-ofthe-Art technique of using encapsulated fluorescent molecular probes (microencapsulated biomarkers) is a unique tool that can serve as a platform for the development of new methods to obtain in vivo physiological measurements and is applicable to a broad range of organisms. Here, we describe a novel technique to monitor the pH of blood inside the gill capillaries and interstitial fluid of muscles by using microencapsulated biomarkers in a zebrafish model. The functionality of the proposed technique is shown by the identification of acidification under anesthesia-induced coma and after death. The pH in muscles reacts to hypoxia faster than that in the gill bloodstream, which makes both parameters applicable as markers of either local or bodily reactions.

Publication DOI:
Divisions: College of Engineering & Physical Sciences > School of Engineering and Technology > Mechanical, Biomedical & Design
College of Engineering & Physical Sciences
Additional Information: © 2017. Published by The Company of Biologists Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Uncontrolled Keywords: Blood ph,Encapsulated fluorescent sensors,Interstitial ph,Microencapsulated biomarkers,Physiological measurements in vivo,Zebrafish,Biochemistry, Genetics and Molecular Biology(all),Agricultural and Biological Sciences(all)
Publication ISSN: 2046-6390
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL) ... content/6/5/673 (Publisher URL)
PURE Output Type: Article
Published Date: 2017-05-15
Accepted Date: 2017-03-28
Authors: Borvinskaya, Ekaterina
Gurkov, Anton
Shchapova, Ekaterina
Baduev, Boris
Shatilina, Zhanna
Sadovoy, Anton
Meglinski, Igor (ORCID Profile 0000-0002-7613-8191)
Timofeyev, Maxim



Version: Published Version

License: Creative Commons Attribution

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