Combined laser speckle imaging and fluorescent intravital microscopy for monitoring acute vascular permeability reaction


Optical clearing agents (OCAs) and many chemicals are widely used in functional diagnosis of skin tissues. Numerous studies are associated with the transcutaneous diffusion of OCA in epidermal, dermal, and hypodermal tissues, which results in changing their optical properties. In addition, an objective approach that is suitable for screening the influence of utilized OCA, as well as various chemical agents, synthetics, and nanomaterials, on blood and lymph flows is highly desirable. In our study, a highly sensitive laser speckle imaging (LSI) system and fluorescent intravital microscopy (FIM) were used team-wise to inspect the acute skin vascular permeability reaction in mouse ear during the local application of OCA on the skin surface. Fluorescent contrast material administrated intravenously was used for quantitatively assessing the intensity of vascular permeability reaction and the strength of skin irritation. The obtained results suggest that a combined use of LSI and FIM is highly effective for monitoring the cutaneous vascular permeability reaction, with great potential for assessment of allergic reactions of skin in response to interactions with chemical substances.

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Divisions: College of Engineering & Physical Sciences > School of Engineering and Technology > Mechanical, Biomedical & Design
College of Engineering & Physical Sciences
Additional Information: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Uncontrolled Keywords: acute vascular reaction,allergens,contact irritant,fluorescence,laser speckle imaging,optical clearing,skin,vascular permeability,Electronic, Optical and Magnetic Materials,Biomaterials,Atomic and Molecular Physics, and Optics,Biomedical Engineering
Publication ISSN: 1560-2281
Last Modified: 20 May 2024 07:31
Date Deposited: 16 Jul 2019 07:28
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://www.spi ... 4.6.060501.full (Publisher URL)
PURE Output Type: Article
Published Date: 2019-05-31
Accepted Date: 2019-04-11
Authors: Kalchenko, Vyacheslav
Meglinski, Igor (ORCID Profile 0000-0002-7613-8191)
Sdobnov, Anton
Kuznetsov, Yuri
Harmelin, Alon



Version: Published Version

License: Creative Commons Attribution

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