A Pilot Study of the Synergy between Two Antimicrobial Peptides and Two Common Antibiotics


Background: Frequent and unrestricted use of antibiotics has been associated with the development of antibiotic resistance by microorganisms. Thus, there is a need to find novel antibacterial agents or a combination of agents as the first line of treatment for various infections. This study aimed to investigate the synergy between antimicrobial peptide (AMP) combinations or between AMP‐antibiotics combinations using two common pathogens, Pseudomonas aeruginosa and Staphylococcus aureus. Methods: The AMPs melimine, Mel4 and protamine, and antibiotics cefepime and ciprofloxacin were used in this study. The minimum inhibitory concentration (MIC) of each were evaluated against P. aeruginosa and S. aureus strains by a microtiter broth dilution. Based on the MIC of each antimicrobial agent, a checkerboard assay was performed to investigate the synergy between them, which was expressed as the fractional inhibitory concentration (FIC). Results: The combination of melimine and ciprofloxacin showed synergistic activity against antibiotic sensitive or resistant strains of P. aeruginosa and with FIC values ≤0.5. Conclusion: Combinations of AMPs and the fluoroquinolone ciprofloxacin is a promising method for reducing resistance to the fluoroquinolone of P. aeruginosa.

Publication DOI: https://doi.org/10.3390/antibiotics8020060
Divisions: College of Health & Life Sciences > School of Optometry > Optometry
College of Health & Life Sciences > School of Optometry > Optometry & Vision Science Research Group (OVSRG)
College of Health & Life Sciences
Additional Information: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Uncontrolled Keywords: Antibiotics,Antimicrobial peptides,Pseudomonas,Staphylococcus,Synergy,Microbiology,Biochemistry,Pharmacology, Toxicology and Pharmaceutics(all),Microbiology (medical),Infectious Diseases,Pharmacology (medical)
Publication ISSN: 2079-6382
Last Modified: 27 Jun 2024 09:56
Date Deposited: 16 May 2019 10:42
Full Text Link:
Related URLs: https://www.mdp ... 079-6382/8/2/60 (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2019-05-09
Published Online Date: 2019-05-09
Accepted Date: 2019-05-05
Authors: Kampshoff, Franziska
Willcox, Mark D.P.
Dutta, Debarun (ORCID Profile 0000-0002-2204-5272)



Version: Published Version

License: Creative Commons Attribution

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