Broad spectrum antimicrobial activity of melimine covalently bound to contact lenses


PURPOSE. To develop a stable antimicrobial contact lens, which is effective against the International Organization for Standardization (ISO) panel microorganisms, Acanthamoeba castellanii and drug resistant strains of Pseudomonas aeruginosa and Staphylococcus aureus. METHODS. Melimine was covalently incorporated into etafilcon A lenses. The amount of peptide present on the lens surface was quantified using amino acid analysis. After coating, the heat stability (121°C), lens surface hydrophobicity (by captive bubble), and in vitro cytotoxicity to mouse L929 cells of the lenses were investigated. Antimicrobial activity against the micro-organisms was evaluated by viable plate count and fluorescence microscopy, measuring the proportion of cell death compared with control lenses with no melimine. RESULTS. The most effective concentration was determined to be 152 ± 44 lg lens-1 melimine on the lens surface. After coating, lenses were relatively hydrophilic and were nontoxic to mammalian cells. The activity remained high after autoclaving (e.g., 3.1, 3.9, 1.2, and 1.0 log inhibition against P. aeruginosa, S. aureus, A. castellanii, and Fusarium solani, respectively). Fluorescence microscopy confirmed significantly reduced (P < 0.001) adhesion of viable bacteria to melimine contact lenses. Viable count confirmed that lenses were active against all the bacteria and fungi from the ISO panel, Acanthamoeba and gave at least 2 log inhibition against all the multidrug resistant S. aureus and P. aeruginosa strains. CONCLUSIONS. Melimine may offer excellent potential for development as a broad spectrum antimicrobial coating for contact lenses, showing activity against all the bacterial and fungal ISO panel microorganisms, Acanthamoeba, and antibiotic resistant strains of P. aeruginosa and S. aureus.

Publication DOI:
Divisions: College of Health & Life Sciences > School of Optometry > Optometry
Additional Information: Copyright 2013 The Association for Research in Vision and Ophthalmology, Inc. Creative Commons Attribution Non-Commercial No Derivatives License
Uncontrolled Keywords: Ophthalmology,Sensory Systems,Cellular and Molecular Neuroscience
Publication ISSN: 0146-0404
Last Modified: 11 Dec 2023 08:19
Date Deposited: 17 Apr 2019 14:58
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL) ... ticleid=2188470 (Publisher URL)
PURE Output Type: Article
Published Date: 2013-01-01
Authors: Dutta, Debarun (ORCID Profile 0000-0002-2204-5272)
Cole, Nerida
Kumar, Naresh
Willcox, Mark D.P.

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