A laboratory assessment of factors that affect bacterial adhesion to contact lenses


Adhesion of pathogenic microbes, particularly bacteria, to contact lenses is implicated in contact lens related microbial adverse events. Various in vitro conditions such as type of bacteria, the size of initial inoculum, contact lens material, nutritional content of media, and incubation period can influence bacterial adhesion to contact lenses and the current study investigated the effect of these conditions on bacterial adhesion to contact lenses. There was no significant difference in numbers of bacteria that adhered to hydrogel etafilcon A or silicone hydrogel senofilcon A contact lenses. Pseudomonas aeruginosa adhered in higher numbers compared to Staphylococcus aureus. Within a genera/species, adhesion of different bacterial strains did not differ appreciably. The size of initial inoculum, nutritional content of media, and incubation period played significant roles in bacterial adhesion to lenses. A set of in vitro assay conditions to help standardize adhesion between studies have been recommended.

Publication DOI: https://doi.org/10.3390/biology2041268
Divisions: College of Health & Life Sciences > School of Optometry > Optometry
Additional Information: This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
Uncontrolled Keywords: Bacterial adhesion,Contact lens,Pseudomonas aeruginosa,Staphylococcus aureus,General Agricultural and Biological Sciences,General Biochemistry,Genetics and Molecular Biology,General Immunology and Microbiology
Publication ISSN: 1741-7007
Last Modified: 27 Jun 2024 09:54
Date Deposited: 17 Apr 2019 14:51
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://www.mdp ... 9-7737/2/4/1268 (Publisher URL)
PURE Output Type: Article
Published Date: 2013-11-01
Authors: Dutta, Debarun (ORCID Profile 0000-0002-2204-5272)
Willcox, Mark D P



Version: Published Version

License: Creative Commons Attribution

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