Isolation of a Δ5-fatty acid desaturase gene from Mortierella alpina


Arachidonic acid (C20:4 Δ5,8,11,14)) is a polyunsaturated fatty acid synthesized by the ?-fatty acid desaturation of di-homo-?-linolenic acid (C20:3 Δ8,11,14)). In mammals, it is known to be a precursor of the prostaglandins and the leukotrienes but it is also accumulated by the filamentous fungus Mortierella alpina. We have isolated a cDNA encoding the Δ5-fatty acid desaturase from M. alpina via a polymerase chain reaction- based strategy using primers designed to the conserved histidine box regions of microsomaL desaturases, and confirmed its function by expression in the yeast Saccharomyces cerevisiae. Analysis of the lipids from the transformed yeast demonstrated the accumulation of arachidonic acid. The M. alpina Δ5- desaturase is the first example of a cloned Δ5-desaturase, and differs from other fungal desaturases previously characterized by the presence of an N- terminal domain related to cytochrome b5.

Publication DOI:
Divisions: College of Engineering & Physical Sciences > School of Infrastructure and Sustainable Engineering > Chemical Engineering & Applied Chemistry
College of Engineering & Physical Sciences > Energy and Bioproducts Research Institute (EBRI)
Additional Information: © The American Society for Biochemistry and Molecular Biology, Inc.
Publication ISSN: 1083-351X
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Related URLs: http://www.jbc. ... nt/273/30/19055 (Publisher URL)
PURE Output Type: Article
Published Date: 1998-07-24
Authors: Michaelson, Louise V.
Lazarus, Colin M.
Griffiths, Gareth (ORCID Profile 0000-0002-7507-6945)
Napier, Johnathan A.
Stobart, A.Keith



Version: Published Version

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