Cardiotrophin-1 activates a distinct form of cardiac muscle cell hypertrophy. Assembly of sarcomeric units in series VIA gp130/leukemia inhibitory factor receptor-dependent pathways


Cardiotrophin-1 (CT-1) was recently isolated by expression cloning based on its ability to induce an increase in cell size in neonatal rat ventricular cardiomyocytes. Sequence similarity data suggested that CT-1 is a novel member of a family of structurally related cytokines sharing the receptor component gp130. The present study documents that gp130 is required for CT-1 signaling in cardiomyocytes, by demonstrating that a monoclonal anti-gp130 antibody completely inhibits c-fos induction by CT-1. Similarly, a leukemia inhibitory factor receptor subunit beta (LIFRbeta) antagonist effectively blocks the CT-1 induction of c-fos, indicating a requirement for LIFRbeta in the hypertrophic response, as well. Upon stimulation with CT-1, both gpl30 and the LIFRbeta are tyrosine-phosphorylated, providing further evidence that CT-1 signals through the gp130/LIFRbeta heterodimer in cardiomyocytes. CT-1 induces a hypertrophic response in cardiomyocytes that is distinct from the phenotype seen after alpha-adrenergic stimulation, both with regard to cell morphology and gene expression pattern. Stimulation with CT-1 results in an increase in cardiac cell size that is characterized by an increase in cell length but no significant change in cell width. Confocal laser microscopy of CT-1 stimulated cells reveals the assembly of sarcomeric units in series rather than in parallel, as seen after alpha-adrenergic stimulation. CT-1 induces a distinct pattern of immediate early genes, and up-regulates the atrial natriuretic factor (ANF) gene, but does not affect skeletal alpha-actin or myosin light chain-2v expression. As evidenced by nuclear run-on transcription assays, both CT-1 and alpha-adrenergic stimulation lead to an increase in ANF gene transcription. Transient transfection analyses document that, in contrast to alpha-adrenergic stimulation, the CT-1 responsive cis-regulatory elements are located outside of the proximal 3 kilobase pairs of the ANF 5'-flanking region. These studies indicate that CT-1 can activate a distinct form of myocardial cell hypertrophy, characterized by the promotion of sarcomere assembly in series, via gpl30/LIFRbeta-dependent signaling pathways.

Publication DOI:
Divisions: College of Health & Life Sciences > School of Biosciences > Cell & Tissue Biomedical Research
College of Health & Life Sciences > Chronic and Communicable Conditions
College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
College of Health & Life Sciences > School of Biosciences
Additional Information: © 1996 by The American Society for Biochemistry and Molecular Biology, Inc.
Uncontrolled Keywords: Actins,Animals,Atrial Natriuretic Factor,Cells, Cultured,Ciliary Neurotrophic Factor,Cytokines,Gene Expression,Genes, fos,Growth Inhibitors,Heart,Humans,Interleukin-6,Leukemia Inhibitory Factor,Leukemia Inhibitory Factor Receptor alpha Subunit,Lymphokines,Mice,Myocardium,Nerve Growth Factors,Nerve Tissue Proteins,Proto-Oncogene Proteins c-fos,Receptors, Cytokine,Receptors, OSM-LIF,Recombinant Fusion Proteins,Sarcomeres,Signal Transduction,Transfection
Publication ISSN: 1083-351X
Last Modified: 14 Jun 2024 07:09
Date Deposited: 20 Mar 2019 09:35
Full Text Link:
Related URLs: http://www.jbc. ... ent/271/16/9535 (Publisher URL)
PURE Output Type: Article
Published Date: 1996-04-19
Authors: Wollert, K C
Taga, T
Saito, M
Narazaki, M
Kishimoto, T
Glembotski, C C
Vernallis, Ann
Heath, J K
Pennica, D
Wood, W I
Chien, K R



Version: Published Version

| Preview

Export / Share Citation


Additional statistics for this record