Mesenchymal stromal cell-mediated neuroprotection and functional preservation of retinal ganglion cells in a rodent model of glaucoma


Background aims: Glaucoma is a leading cause of irreversible blindness involving loss of retinal ganglion cells (RGC). Mesenchymal stromal cells (MSC) have shown promise as a paracrine-mediated therapy for compromised neurons. It is, however, unknown whether dental pulp stem cells (DPSC) are effective as a cellular therapy in glaucoma and how their hypothesized influence compares with other more widely researched MSC sources. The present study aimed to compare the efficacy of adipose-derived stem cells, bone marrow-derived MSC (BMSC) and DPSC in preventing the loss of RGC and visual function when transplanted into the vitreous of glaucomatous rodent eyes. Methods: Thirty-five days after raised intraocular pressure (IOP) and intravitreal stem cell transplantation, Brn3a+ RGC numbers, retinal nerve fibre layer thickness (RNFL) and RGC function were evaluated by immunohistochemistry, optical coherence tomography and electroretinography, respectively. Results: Control glaucomatous eyes that were sham-treated with heat-killed DPSC had a significant loss of RGC numbers, RNFL thickness and function compared with intact eyes. BMSC and, to a greater extent, DPSC provided significant protection from RGC loss and RNFL thinning and preserved RGC function. Discussion: The study supports the use of DPSC as a neuroprotective cellular therapy in retinal degenerative disease such as glaucoma.

Publication DOI:
Divisions: College of Health & Life Sciences > Aston Medical School
Additional Information: © 2015, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Uncontrolled Keywords: Dental pulp stem cells,Glaucoma,Mesenchymal stromal cells,Neuroprotection,Retinal ganglion cells,Stem cell transplantation,Immunology and Allergy,Immunology,Oncology,Genetics(clinical),Cell Biology,Transplantation,Cancer Research
Publication ISSN: 1477-2566
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://www.sci ... 1639?via%3Dihub (Publisher URL)
PURE Output Type: Article
Published Date: 2016-04-01
Published Online Date: 2016-02-17
Accepted Date: 2015-12-16
Authors: Mead, Ben
Hill, Lisa J.
Blanch, Richard J.
Ward, Kelly
Logan, Ann
Berry, Martin
Leadbeater, Wendy (ORCID Profile 0000-0003-3141-9421)
Scheven, Ben A.

Export / Share Citation


Additional statistics for this record