Hollow-fiber membrane technology: Characterization and proposed use as a potential mimic of skin vascularization towards the development of a novel skin absorption in vitro model


Dermal bioavailability is currently estimated through skin penetration studies using ex vivo models, which lack any measure of capillary bed function, and thus do not fully reproduce physiological conditions. We propose a novel strategy to mimic skin vascularization using newly fabricated hollow fibers made from a biocompatible membrane material, polystyrene, which is hydrophobic if left untreated, or hydrophilic when its surface polarity is modified through plasma-treatment. Caffeine has been well studied in skin penetration assays and was used here to determine the permeation properties of the hollow fibers in a novel jacketed glass bioreactor. For hydrophobic fibers, approximately 87.2 % of the caffeine dose did not penetrate the porous surface; 0.2 % of the dose was collected after 24 h (permeated through the pores), and therefore 12.6 % of the initial dose was suspected to block the membrane. For hydrophilic fibers, both the percentage of the initial dose that permeated and that of blocking caffeine increased to 1.2 % and 35.2 % respectively. It was concluded that caffeine permeated the hollow fibers at similar times of clearance to those observed in vivo, and therefore shows that this new model could provide a surrogate for capillary-based clearance in in vitro skin absorption studies.

Publication DOI: https://doi.org/10.1016/j.bej.2019.01.025
Divisions: College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences > Cell & Tissue Biomedical Research
Additional Information: © 2019, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords: Chemical adsorption,Hollow fiber membranes,Skin penetration,Systemic toxicity,Tissue engineering,Biotechnology,Bioengineering,Environmental Engineering,Biomedical Engineering
Publication ISSN: 1873-295X
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Related URLs: https://www.sci ... 0397?via%3Dihub (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2019-05-15
Published Online Date: 2019-01-30
Accepted Date: 2019-01-30
Authors: Esteban, Patricia Perez (ORCID Profile 0000-0001-7084-1065)
Pickles, Juliette
Scott, Andrew D.
Ellis, Marianne J.

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