Real-time polarimetric biosensing using macroporous alumina membranes


We report the first demonstration of real-time biosensing in free standing macroporous alumina membranes. The membranes with their 200 nm diameter pores are ideal candidates for biosensing applications where fast response times for small sample volumes are needed as they allow analytes to flow through the pores close to the bioreceptors immobilized on the pores walls. A bulk refractive index sensitivity of 5.2×10-6 refractive index units was obtained from signal responses to different concentrations of NaCl solutions flowing through the pores. Finally, after functionalizing the alumina pore surfaces with an epoxysilane and then spotting it with β-Lactoglobulin protein, the interactions between the β-lactoglobulin and rabbit anti-β-lactoglobulin, as well as the interaction between the rabbit anti-β-lactoglobulin and a secondary antibody anti-rabbit Immunoglobulin G were monitored in real-time.

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Divisions: College of Engineering & Physical Sciences > Aston Institute of Photonics Technology (AIPT)
Additional Information: Copyright 2013 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Uncontrolled Keywords: Biological sensing and sensors,Form birefringence,Optical sensing and sensors,Polarimetry,Porous alumina,Atomic and Molecular Physics, and Optics,Electronic, Optical and Magnetic Materials,Biomaterials,Radiology Nuclear Medicine and imaging
Publication ISSN: 1996-756X
Last Modified: 08 Jan 2024 08:59
Date Deposited: 18 Feb 2019 14:35
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://www.spi ... 7432.full?SSO=1 (Publisher URL)
PURE Output Type: Conference article
Published Date: 2013-05-28
Authors: Álvarez, Jesús
Sola, Laura
Platt, Geoff
Cretich, Marina
Swann, Marcus
Chiari, Marcella
Hill, Daniel (ORCID Profile 0000-0003-3510-7783)
Martinez-Pastor, Juan



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