Real time optical immunosensing with flow-through porous alumina membranes


Through the presentation of analytical data from bioassay experiments, measured by polarimetry, we demonstrate for the first time a real time immunoassay within a free standing macroporous alumina membrane. The 200 nm nominal pore diameter of the membrane enables flow-through, thereby providing an ideal fluidic platform for the targeted delivery of analytes to bioreceptors immobilized on the pore walls, enabling fast sensing response times and the use of small sample volumes (<100 μL). For the immunoassay, the pore walls were first coated with the functional copolymer, copoly(DMA-NAS) using a novel coupling process, before immobilization of the allergen protein, β-lactoglobulin, by spotting. The immuno-assay then proceeded with the binding of the primary and secondary antibody cognates, rabbit anti-β-lactoglobulin and anti-rabbit IgG respectively. Through the use of streptavidin coated quantum dots as refractive index signal enhancers, a noise floor for individual measurements of 3.7 ng/mL (25 pM) was obtained, with an overall statistical, or formal assay LOD of 33.7 ng/mL (225 pM), for total assay time below 1 h.

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Divisions: College of Engineering & Physical Sciences > Aston Institute of Photonics Technology (AIPT)
Funding Information: The authors would like to acknowledge the contributions from Geoff Platt (Farfield), Isabelle Metton (Phylogene), Steven Sievers (Charité) and Thomas Stadelmann (CSEM) for their participating in prior biosensing experiments that led up to this work. We al
Additional Information: © 2014, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Uncontrolled Keywords: Copolymer,Form birefringence,Optical biosensing,Polarimetry,Porous alumina,Quantum dots,Electronic, Optical and Magnetic Materials,Instrumentation,Condensed Matter Physics,Surfaces, Coatings and Films,Metals and Alloys,Materials Chemistry,Electrical and Electronic Engineering
Publication ISSN: 0925-4005
Last Modified: 27 May 2024 07:27
Date Deposited: 15 Feb 2019 16:36
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://www.sci ... 7163?via%3Dihub (Publisher URL)
PURE Output Type: Article
Published Date: 2014-10-31
Accepted Date: 2014-06-08
Authors: Álvarez, Jesús
Sola, Laura
Cretich, Marina
Swann, Marcus J.
Gylfasson, Kristinn B.
Volden, Tormod
Chiari, Marcella
Hill, Daniel (ORCID Profile 0000-0003-3510-7783)

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