Dr1 (NC2) is present at tRNA genes and represses their transcription in human cells


Dr1 (also known as NC2β) was identified as a repressor of RNA polymerase (pol) II transcription. It was subsequently shown to inhibit pol III transcription when expressed at high levels in vitro or in yeast cells. However, endogenous Dr1 was not detected at pol III-transcribed genes in growing yeast. In contrast, we demonstrate that endogenous Dr1 is present at pol III templates in human cells, as is its dimerization partner DRAP1 (also called NC2α). Expression of tRNA by pol III is selectively enhanced by RNAi-mediated depletion of endogenous human Dr1, but we found no evidence that DRAP1 influences pol III output in vivo. A stable association was detected between endogenous Dr1 and the pol III-specific transcription factor Brf1. This interaction may recruit Dr1 to pol III templates in vivo, as crosslinking to these sites increases following Brf1 induction. On the basis of these data, we conclude that the physiological functions of human Dr1 include regulation of pol III transcription.

Publication DOI: https://doi.org/10.1093/nar/gkp1102
Divisions: College of Health & Life Sciences > Aston Medical School
Additional Information: © The Author(s) 2009. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords: Genetics
Publication ISSN: 1362-4962
Last Modified: 08 Jan 2024 08:48
Date Deposited: 13 Nov 2018 15:31
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://academi ... 112460#82650055 (Publisher URL)
PURE Output Type: Article
Published Date: 2009-12-03
Authors: Kantidakis, Theodoros (ORCID Profile 0000-0002-3515-5235)
White, Robert J.


Export / Share Citation


Additional statistics for this record