Comprehensive in vitro and in vivo studies of novel melt-derived Nb-substituted 45S5 bioglass reveal its enhanced bioactive properties for bone healing


The present work presents and discusses the results of a comprehensive study on the bioactive properties of Nb-substituted silicate glass derived from 45S5 bioglass. In vitro and in vivo experiments were performed. We undertook three different types of in vitro analyses: (i) investigation of the kinetics of chemical reactivity and the bioactivity of Nb-substituted glass in simulated body fluid (SBF) by 31P MASNMR spectroscopy, (ii) determination of ionic leaching profiles in buffered solution by inductively coupled plasma optical emission spectrometry (ICP-OES), and (iii) assessment of the compatibility and osteogenic differentiation of human embryonic stem cells (hESCs) treated with dissolution products of different compositions of Nb-substituted glass. The results revealed that Nb-substituted glass is not toxic to hESCs. Moreover, adding up to 1.3 mol% of Nb2O5 to 45S5 bioglass significantly enhanced its osteogenic capacity. For the in vivo experiments, trial glass rods were implanted into circular defects in rat tibia in order to evaluate their biocompatibility and bioactivity. Results showed all Nb-containing glass was biocompatible and that the addition of 1.3 mol% of Nb2O5, replacing phosphorous, increases the osteostimulation of bioglass. Therefore, these results support the assertion that Nb-substituted glass is suitable for biomedical applications.

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Divisions: College of Engineering & Physical Sciences > Aston Institute of Materials Research (AIMR)
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Publication ISSN: 2045-2322
Last Modified: 17 Jul 2024 07:07
Date Deposited: 29 Aug 2018 08:06
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Related URLs: https://www.nat ... 598-018-31114-0 (Publisher URL)
PURE Output Type: Article
Published Date: 2018-08-24
Accepted Date: 2018-08-02
Authors: Souza, Lucas (ORCID Profile 0000-0002-0188-5168)
Lopes, João Henrique
Encarnação, Davi
Mazali, Italo Odone
Martin, Richard Alan (ORCID Profile 0000-0002-6013-2334)
Camilli, José Angelo
Bertran, Celso Aparecido



Version: Published Version

License: Creative Commons Attribution

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