Souza, Lucas, Lopes, João Henrique, Encarnação, Davi, Mazali, Italo Odone, Martin, Richard Alan, Camilli, José Angelo and Bertran, Celso Aparecido (2018). Comprehensive in vitro and in vivo studies of novel melt-derived Nb-substituted 45S5 bioglass reveal its enhanced bioactive properties for bone healing. Scientific Reports, 8 (1),
Abstract
The present work presents and discusses the results of a comprehensive study on the bioactive properties of Nb-substituted silicate glass derived from 45S5 bioglass. In vitro and in vivo experiments were performed. We undertook three different types of in vitro analyses: (i) investigation of the kinetics of chemical reactivity and the bioactivity of Nb-substituted glass in simulated body fluid (SBF) by 31P MASNMR spectroscopy, (ii) determination of ionic leaching profiles in buffered solution by inductively coupled plasma optical emission spectrometry (ICP-OES), and (iii) assessment of the compatibility and osteogenic differentiation of human embryonic stem cells (hESCs) treated with dissolution products of different compositions of Nb-substituted glass. The results revealed that Nb-substituted glass is not toxic to hESCs. Moreover, adding up to 1.3 mol% of Nb2O5 to 45S5 bioglass significantly enhanced its osteogenic capacity. For the in vivo experiments, trial glass rods were implanted into circular defects in rat tibia in order to evaluate their biocompatibility and bioactivity. Results showed all Nb-containing glass was biocompatible and that the addition of 1.3 mol% of Nb2O5, replacing phosphorous, increases the osteostimulation of bioglass. Therefore, these results support the assertion that Nb-substituted glass is suitable for biomedical applications.
| Publication DOI: | https://doi.org/10.1038/s41598-018-31114-0 |
|---|---|
| Divisions: | College of Engineering & Physical Sciences > Aston Institute of Materials Research (AIMR) |
| Additional Information: | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2018 |
| Publication ISSN: | 2045-2322 |
| Last Modified: | 20 Dec 2024 08:11 |
| Date Deposited: | 29 Aug 2018 08:06 |
| Full Text Link: | |
| Related URLs: |
https://www.nat ... 598-018-31114-0
(Publisher URL) |
PURE Output Type: | Article |
| Published Date: | 2018-08-24 |
| Accepted Date: | 2018-08-02 |
| Authors: |
Souza, Lucas
(
0000-0002-0188-5168)
Lopes, João Henrique Encarnação, Davi Mazali, Italo Odone Martin, Richard Alan (
0000-0002-6013-2334)
Camilli, José Angelo Bertran, Celso Aparecido |
CORE (COnnecting REpositories)
CORE (COnnecting REpositories)