S100P enhances the motility and invasion of human trophoblast cell lines


S100P has been shown to be a marker for carcinogenesis where its expression in solid tumours correlates with metastasis and a poor patient prognosis. This protein’s role in any physiological process is, however, unknown. Here we first show that S100P is expressed both in trophoblasts in vivo as well as in some corresponding cell lines in culture. We demonstrate that S100P is predominantly expressed during the early stage of placental formation with its highest expression levels occurring during the first trimester of gestation, particularly in the invading columns and anchoring villi. Using gain or loss of function studies through overexpression or knockdown of S100P expression respectively, our work shows that S100P stimulates both cell motility and cellular invasion in different trophoblastic and first trimester EVT cell lines. Interestingly, cell invasion was seen to be more dramatically affected than cell migration. Our results suggest that S100P may be acting as an important regulator of trophoblast invasion during placentation. This finding sheds new light on a hitherto uncharacterized molecular mechanism which may, in turn, lead to the identification of novel targets that may explain why significant numbers of confirmed human pregnancies suffer complications through poor placental implantation.

Publication DOI: https://doi.org/10.1038/s41598-018-29852-2
Divisions: College of Health & Life Sciences
College of Health & Life Sciences > Aston Medical School
College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
Additional Information: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2018
Publication ISSN: 2045-2322
Last Modified: 17 Jun 2024 07:33
Date Deposited: 27 Jun 2018 08:45
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Related URLs: https://www.nat ... 598-018-29852-2 (Publisher URL)
PURE Output Type: Article
Published Date: 2018-07-31
Accepted Date: 2018-06-26
Authors: Ebrahimzadeh Asl Tabrizi, Maral (ORCID Profile 0000-0002-0580-7933)
Lancaster, Tara
Ismail, Thamir M.
Ganguly, Ankana
Georgiadou, Athina
Wang, Keqing (ORCID Profile 0000-0001-6239-6344)
Rudland, Philip S.
Ahmad, Shakil S (ORCID Profile 0000-0002-9294-0475)
Gross, Stephane R (ORCID Profile 0000-0002-0867-8866)



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