Chromatography of oxysterols

Abstract

Oxysterols play important roles in development and diseases, but can be highly challenging to analyze. To ensure satisfactory measurements, oxysterols must typically be separated with chromatography prior to detection. Here, we will devote attention to the chromatography of oxysterols, focusing on gas chromatography and liquid chromatography. We will present the role of stationary phases, mobile phases, and dimensions and geometries of particles/columns. We discuss how these parameters may affect the chromatography, regarding factors such as speed and resolution. Finally, we present some less explored avenues for separation of oxysterols.

Publication DOI: https://doi.org/10.1016/j.biochi.2018.05.004
Divisions: College of Health & Life Sciences
College of Health & Life Sciences > Chronic and Communicable Conditions
College of Health & Life Sciences > School of Psychology
College of Health & Life Sciences > School of Biosciences > Cell & Tissue Biomedical Research
Additional Information: © 2018, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Funding: Kidney Research Foundation PDF3/2014 and Alzheimer's Research UK network grants.
Uncontrolled Keywords: (max 6)Oxysterols,Chromatography,Separation,Lipids,Sterols
Publication ISSN: 0300-9084
Last Modified: 29 Apr 2024 07:24
Date Deposited: 09 May 2018 07:12
Full Text Link:
Related URLs: https://www.sci ... 300908418301275 (Publisher URL)
PURE Output Type: Review article
Published Date: 2018-10
Published Online Date: 2018-05-09
Accepted Date: 2018-05-04
Authors: Dias, Irundika H.k. (ORCID Profile 0000-0002-6620-8221)
Wilson, Steven R.
Roberg-larsen, Hanne

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