A pilot study using laser-based technique for non-invasive diagnostics of hypertensive conditions in mice

Abstract

Endothelial dysfunction is directly linked to preeclampsia, a maternal hypertensive condition that is life threating for both the mother and the baby. Epidemiological studies show that women with a history of pre-eclampsia have an elevated risk for cardiovascular disease. Here we report a new non-invasive diagnostic test for preeclampsia in mice that allows us to non-invasively assess the condition of the animals during the experiment and treatment in established models of preeclampsia. A laser-based multifunctional diagnostics system (LAKK-M) was chosen to carry out non-invasive analysis of multiple parameters. The device was used to simultaneously record the microcirculatory blood flow and oxygen saturation, as well as fluorescence levels of endogenous fluorophores. Preliminary experiments were conducted on adenoviral (Ad-)- mediated overexpression of sFlt-1 (Ad-sFlt-1) to mimic preeclampsialike symptoms in mice. The recorded data displayed the ability of the LAKK-M diagnostics device to detect significant differences in perfusion measurements between the control and Ad-sFlt-1 treatment. Preliminary results provide a potential avenue to employ these diagnostics technology to monitor and aid in maintaining control of live animal conditions throughout the experiment and treatment.

Publication DOI: https://doi.org/10.1117/12.2213026
Divisions: College of Engineering & Physical Sciences
College of Health & Life Sciences > Aston Medical School
College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
College of Engineering & Physical Sciences > Aston Institute of Photonics Technology (AIPT)
Aston University (General)
Additional Information: © (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).
Publication ISSN: 1996-756X
Last Modified: 03 Dec 2024 08:17
Date Deposited: 15 Feb 2018 11:50
Full Text Link: http://gateway. ... 000377396200094
Related URLs: https://www.spi ... 2.2213026.short (Publisher URL)
PURE Output Type: Conference article
Published Date: 2016-02-29
Accepted Date: 2016-02-28
Authors: Litvinova, Karina S. (ORCID Profile 0000-0003-1110-8589)
Ahmad, Shakil (ORCID Profile 0000-0002-9294-0475)
Wang, Keqing (ORCID Profile 0000-0001-6239-6344)
Rafailov, Ilya E.
Sokolovski, Sergei G. (ORCID Profile 0000-0001-7445-7204)
Zhang, Lin (ORCID Profile 0000-0002-1691-5577)
Rafailov, Edik U. (ORCID Profile 0000-0002-4152-0120)
Ahmed, Asif (ORCID Profile 0000-0002-8755-8546)

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