Use of a ceramic membrane to improve the performance of two-separate-phase biocatalytic membrane reactor


Biocatalytic membrane reactors (BMR) combining reaction and separation within the same unit have many advantages over conventional reactor designs. Ceramic membranes are an attractive alternative to polymeric membranes in membrane biotechnology due to their high chemical, thermal and mechanical resistance. Another important use is their potential application in a biphasic membrane system, where support solvent resistance is highly needed. In this work, the preparation of asymmetric ceramic hollow fibre membranes and their use in a two-separate-phase biocatalytic membrane reactor will be described. The asymmetric ceramic hollow fibre membranes were prepared using a combined phase inversion and sintering technique. The prepared fibres were then used as support for lipase covalent immobilization in order to develop a two-separate-phase biocatalytic membrane reactor. A functionalization method was proposed in order to increase the density of the reactive hydroxyl groups on the surface of ceramic membranes, which were then amino-activated and treated with a crosslinker. The performance and the stability of the immobilized lipase were investigated as a function of the amount of the immobilized biocatalytst. Results showed that it is possible to immobilize lipase on a ceramic membrane without altering its catalytic performance (initial residual specific activity 93%), which remains constant after 6 reaction cycles.

Publication DOI:
Divisions: College of Engineering & Physical Sciences
College of Engineering & Physical Sciences > Aston Institute of Materials Research (AIMR)
College of Engineering & Physical Sciences > Energy and Bioproducts Research Institute (EBRI)
Additional Information: © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license ( Funding: project PON01_01545, Olio più, within the framework PON Ricerca e Competitività 2007–2013.
Uncontrolled Keywords: Biocatalytic membrane,Ceramic biocatalytic membrane,Immobilized lipase,Two-separate-phase biocatalytic membrane reactor,Medicine(all),Organic Chemistry
Publication ISSN: 1420-3049
Last Modified: 16 May 2024 07:11
Date Deposited: 08 Feb 2018 08:51
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2016-03-08
Accepted Date: 2016-03-04
Authors: Ranieri, Giuseppe
Mazzei, Rosalinda
Wu, Zhentao (ORCID Profile 0000-0002-4934-8046)
Li, Kang
Giorno, Lidietta



Version: Published Version

License: Creative Commons Attribution

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