Technical challenges of working with extracellular vesicles


Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.

Publication DOI:
Divisions: College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences > School of Biosciences > Cell & Tissue Biomedical Research
College of Health & Life Sciences > Chronic and Communicable Conditions
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
Additional Information: This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Uncontrolled Keywords: Materials Science(all)
Publication ISSN: 2040-3372
Last Modified: 08 Apr 2024 07:19
Date Deposited: 01 Feb 2018 09:55
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
http://pubs.rsc ... 0b#!divAbstract (Publisher URL)
PURE Output Type: Review article
Published Date: 2017-12-13
Published Online Date: 2017-12-13
Accepted Date: 2017-12-08
Authors: Ramirez, Marcel I.
Amorim, Maria G.
Gadelha, Catarina
Milic, Ivana (ORCID Profile 0000-0001-7531-7561)
Welsh, Joshua A.
Freitas, Vanessa M.
Nawaz, Muhammad
Akbar, Naveed
Couch, Yvonne
Makin, Laura
Cooke, Fiona
Vettore, Andre L.
Batista, Patricia X.
Freezor, Roberta
Pezuk, Julia A.
Rosa-Fernandes, Lívia
Carreira, Ana Claudia O.
Devitt, Andrew (ORCID Profile 0000-0002-4651-6761)
Jacobs, Laura
Silva, Israel T.
Coakley, Gillian
Nunes, Diana N.
Carter, Dave
Palmisano, Giuseppe
Dias-Neto, Emmanuel



Version: Published Version

License: Creative Commons Attribution

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