Lipase immobilised on silica monoliths as continuous-flow microreactors for triglyceride transesterification

Abstract

Lipase immobilised on silica monoliths has been prepared and applied as biocatalytic continuous-flow microreactors for the transesterification of tributyrin as a model bio-oil component. Candida antarctica lipase was trapped within the pores of silica monoliths, and its successful immobilisation was demonstrated by the hydrolysis of 4-nitrophenyl butyrate to 4-nitrophenol. Lipase immobilised on silica monoliths was active for the transesterification of tributyrin at ambient temperature, with reactivity as a function of the methanol : tributyrin ratio, flow rate, temperature, and textural properties. Monoliths with a high surface area and large meso- and macropore channels enhanced the transesterification activity through improved molecule diffusion. The optimum immobilised lipase microreactor exhibited almost quantitative ester production for >100 h at 30 °C without deactivation

Publication DOI: https://doi.org/10.1039/C7RE00162B
Divisions: College of Engineering & Physical Sciences
College of Engineering & Physical Sciences > Energy and Bioproducts Research Institute (EBRI)
Additional Information: © Royal Society of Chemistry, 2017. Lipase immobilised on silica monoliths as continuous-flow microreactors for triglyceride transesterification Alotaibi, M., Manayil, J. C., Greenway, G. M., Haswell, S. J., Kelly, S. M., Lee, A. F., Wilson, K. & Kyriakou, G. 18 Dec 2017 In : Reaction Chemistry & Engineering. http://doi.org/10.1039/C7RE00162B
Publication ISSN: 2058-9883
Last Modified: 18 Jun 2024 07:17
Date Deposited: 23 Jan 2018 08:17
Full Text Link:
Related URLs: https://www.sco ... 30ab1b423af66f1 (Scopus URL)
http://xlink.rs ... ?DOI=C7RE00162B (Publisher URL)
PURE Output Type: Article
Published Date: 2018
Published Online Date: 2017-12-18
Accepted Date: 2017-12-18
Authors: Alotaibi, Mohammed
Manayil, Jinesh C. (ORCID Profile 0000-0002-9864-3332)
Greenway, Gillian M.
Haswell, Stephen J.
Kelly, Stephen M.
Lee, Adam F.
Wilson, Karen
Kyriakou, Georgios

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