Neurotensin receptor 1 facilitates intracellular and transepithelial delivery of macromolecules

Abstract

G protein-coupled receptors are expressed on the surface of eukaryotic cells and internalise in response to ligand binding. The actions of the hormone and neurotransmitter neurotensin (NT) are predominantly mediated by specific interactions with one such receptor. Neurotensin receptor 1 (NTS1), which is upregulated in a variety of cancers, including pancreatic and breast tumours. NTS1 could therefore serve as a target for selective delivery of therapeutics. This study characterised the expression of NTS1 in HEK293 cells, as well as both polarised and non-polarised intestinal epithelial Caco-2 cells. NT-conjugated fluorophores were internalised in NTS1-expressing HEK293 and Caco-2 cells in a receptor-mediated fashion. Confocal microscopy revealed fluorophore localisation in the perinuclear region. Cell uptake and transport across the Caco-2 intestinal model of two NT-conjugated fluorophores (GFP and fluorescein) were compared to evaluate the effect of cargo size on cellular uptake. This work demonstrates that NT ligand conjugation is able to deliver relatively large macromolecular cargoes selectively into cells overexpressing NTS1 and the system is able to effectively translocate macromolecules across an intestinal epithelial model. NTS1 therefore shows potential as a drug delivery target not only for targeted but also non-invasive (oral) delivery of biotherapeutics for cancer.

Publication DOI: https://doi.org/10.1016/j.ejpb.2017.06.027
Divisions: College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences
Additional Information: © 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Uncontrolled Keywords: Journal Article
Publication ISSN: 1873-3441
Last Modified: 18 Mar 2024 08:21
Date Deposited: 07 Sep 2017 09:10
Full Text Link:
Related URLs: https://www.sci ... 0449?via%3Dihub (Publisher URL)
PURE Output Type: Article
Published Date: 2017-10-01
Published Online Date: 2017-07-06
Accepted Date: 2017-06-28
Authors: Bird, Joanna L.
Simpson, Rachael
Vllasaliu, Driton
Goddard, Alan D. (ORCID Profile 0000-0003-4950-7470)

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