Label-free glucose biosensor based on enzymatic graphene oxide-functionalized tilted fiber grating


A label-free biosensor based on graphene oxide (GO) and glucose oxidase (GOD) functionalized tilted fiber grating (TFG) with large tilted angle is proposed for low concentration glucose detection. Taking advantages of sufficient binding sites of the GO with oxygen-containing groups, the enzymes (GOD) are covalently immobilized onto GO-deposited TFG via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxyl succinimide cross-liner. Surface characterizations with optical microscopy, scanning electron microscopy, Raman and infrared spectroscopy provide detailed assessments and evidences about the homogeneity of GO deposition and the effectiveness of enzyme modification. Through the specific catalysis reaction of GOD on the glucose, a considerable refractive index change in local microenvironment around the TFG results in the resonant wavelength shifts of cladding modes. The detection results of the low-concentration glucose demonstrate that the resonant wavelength has a linear response to the glucose concentration in the range of 0–8 mM with a response coefficient of ∼0.24 nm/mM, showing an enhanced sensitivity and bio-selectivity compared with the pristine TFG. The miniaturized size and remote label-free sensing capacity of the proposed device permit a multitude of opportunities for single-point measurement in harsh conditions and hard-to-reach spaces, presenting a promising candidate for label-free glucose detection for disease diagnosis, pharmaceutical research and bioengineering applications.

Publication DOI:
Divisions: College of Engineering & Physical Sciences
College of Engineering & Physical Sciences > Aston Institute of Photonics Technology (AIPT)
College of Engineering & Physical Sciences > School of Infrastructure and Sustainable Engineering > Chemical Engineering & Applied Chemistry
College of Engineering & Physical Sciences > Energy and Bioproducts Research Institute (EBRI)
Additional Information: © 2017, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Uncontrolled Keywords: biosensor,Fiber grating,glucose,glucose oxidase,graphene oxide,optical fiber,Electronic, Optical and Magnetic Materials,Instrumentation,Condensed Matter Physics,Surfaces, Coatings and Films,Metals and Alloys,Materials Chemistry,Electrical and Electronic Engineering
Publication ISSN: 0925-4005
Last Modified: 20 May 2024 07:20
Date Deposited: 31 Aug 2017 10:15
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://www.sci ... 3175?via%3Dihub (Publisher URL)
PURE Output Type: Article
Published Date: 2018-01-01
Published Online Date: 2017-07-18
Accepted Date: 2017-07-14
Authors: Jiang, Biqiang
Zhou, Kaiming (ORCID Profile 0000-0002-6011-1912)
Wang, Changle
Sun, Qizhen
Yin, Guolu
Tai, Zhijun
Wilson, Karen (ORCID Profile 0000-0003-4873-708X)
Zhao, Jianlin
Zhang, Lin (ORCID Profile 0000-0002-1691-5577)

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