Cartwright, Stephanie, Bill, Roslyn M., Bui, Sy Tien, Van, Hieu Tran and Nguyen, Hung Minh (2017). Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris. BMC Research Notes, 10 ,
Abstract
Objective: Patients with dual hepatitis B (HBV) and hepatitis D (HDV) virus infection are at an increased risk of progression to liver cirrhosis and hepatocellular carcinoma than patients with a single viral infection. Treatment of viral hepatitis due to dual HBV/HDV infection represents a challenge. Currently there is no vaccine against HDV. Recombinant production of HDV antigen (HDAg) is the first step towards a potential vaccine candidate and the development of assays for HDV detection. Results: This study demonstrates the expression of one HDAg isoform, S-HDAg, in Pichia pastoris. A recombinant vector carrying a tagged gene encoding S-HDAg under the control of the methanol-inducible promoter AOX1 was designed and integrated into P. pastoris X33. The protein, which was purified using a Ni2+ affinity column and eluted at 100-150 mM imidazole, has potential as a recombinant antigen for further study.
Publication DOI: | https://doi.org/10.1186/s13104-017-2692-8 |
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Divisions: | College of Health & Life Sciences College of Health & Life Sciences > School of Biosciences College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine |
Additional Information: | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
Uncontrolled Keywords: | HDAg,hepatitis delta virus,pichia pastoris,protein expression,General Medicine,General Biochemistry,Genetics and Molecular Biology |
Publication ISSN: | 1756-0500 |
Last Modified: | 02 Oct 2024 07:17 |
Date Deposited: | 28 Jul 2017 12:30 |
Full Text Link: | |
Related URLs: |
http://www.scop ... tnerID=8YFLogxK
(Scopus URL) |
PURE Output Type: | Article |
Published Date: | 2017-07-27 |
Accepted Date: | 2017-07-24 |
Authors: |
Cartwright, Stephanie
Bill, Roslyn M. ( 0000-0003-1331-0852) Bui, Sy Tien Van, Hieu Tran Nguyen, Hung Minh |