Membrane emulsification for the production of uniform poly-N-isopropylacrylamide-coated alginate particles using internal gelation


Alginate particles, crosslinked by calcium ions, have a number of potential biopharmaceutical industry applications due to the biocompatibility of the materials used and formed. One such use is as microcarriers for cell attachment,growth and then detachment without the use of proteolytic enzymes. A straightforward and reproducible method for producing uniform calcium alginate particles with controllable median diameters which employs membrane emulsification and internal gelation (solid particles contained in the dispersed phase) is demonstrated, as well as functionalisation of the resulting beads with amine terminated poly N-isopropylacrylamide (pNIPAM) to form temperature responsive particles, by taking advantage of the electrostatic interaction between the carboxyl groups of the alginate and amino groups of the modified pNIPAM. Cell attachment, growth and detachment capabilities of these core–shell structures were assessed and successfully demonstrated by using phase contrast microscopy and fluorescent staining with calcein-AM and ethidium homodimer-1.The formulation used for the alginate particles avoided non-GRAS chemicals by only using food grade and pharmaceutical grade reagents. The median particle size was controllable within the range between 55 m and 690 m and the size distributions produced were very narrow: ‘span’ values as low as 0.2. When using a membrane pore sizeof 20 m no membrane blockage by the suspended calcium carbonate necessary for internal gelation of the alginate particles was observed. Membrane pore openings with diameters of 5 and 10 m were also tested, but blocked with the 2.3 m median diameter calcium carbonate solids.

Publication DOI:
Divisions: College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences > Cell & Tissue Biomedical Research
Additional Information: © 2013, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Uncontrolled Keywords: pNIPAM,microcarriers,microbeads,alginate,cell release,Chemical Engineering(all)
Publication ISSN: 1744-3563
Last Modified: 04 Apr 2024 07:11
Date Deposited: 25 May 2017 09:10
PURE Output Type: Article
Published Date: 2014-09
Published Online Date: 2013-12-16
Accepted Date: 2013-12-09
Submitted Date: 2013-09-17
Authors: Hanga, Mariana Petronela (ORCID Profile 0000-0002-2427-4052)
Holdich, Richard G.

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