Gu, Junzhong, Zhang, Huiyuan, Ji, Bingyang, Jiang, Hui, Zhao, Tao, Jiang, Rongcai, Zhang, Zhiren, Tan, Shengjiang, Ahmed, Asif and Gu, Yuchun (2017). Vesicle miR-195 derived from endothelial cells inhibits expression of serotonin transporter in vessel smooth muscle cells. Scientific Reports, 7 ,
Abstract
Serotonin or 5-hydroxytryptamine (5-HT) has been shown to be essential in lots of physiological and pathological processes. It is well known that 5-HT and 5-HT transporter (5-HTT) play important roles in the pulmonary artery in pulmonary hypertension. However, little is known about the function of 5-HTT in other arteries. In this study we found that the expression of 5-HTT was elevated in injured carotid arteries and over-expression of 5-HTT induced proliferation of smooth muscle cells (SMCs); however, this phenotype could be reversed by knocking-down of 5-HTT or endothelial cells conditional medium (EC-CM). A 5-HTT inhibitor, fluoxetine, treated animals also exhibited reduced restenosis after injury. We identified that miR-195 was packaged in the extracellular vesicles from EC-CM. We further confirmed that extracellular vesicles could transfer miR-195 from ECs to SMCs to inhibit the expression of 5-HTT in SMCs and the proliferation of SMCs. These results provide the first evidence that ECs communicate with SMCs via micro-RNA195 in the regulation of the proliferation of SMCs through 5-HTT, which will contribute to a better understanding of communications between ECs and SMCs via micro-RNA. Our findings suggest a potential target for the control of vessel restenosis.
Publication DOI: | https://doi.org/10.1038/srep43546 |
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Divisions: | College of Health & Life Sciences > Aston Medical School |
Additional Information: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ Funding: 973 Project No. 2013CB531206, 973 Project No. 2012CB517803 and NSF No.81170236, No.31127001 |
Uncontrolled Keywords: | General |
Publication ISSN: | 2045-2322 |
Last Modified: | 11 Dec 2024 08:10 |
Date Deposited: | 04 Apr 2017 13:45 |
Full Text Link: | |
Related URLs: |
http://www.scop ... tnerID=8YFLogxK
(Scopus URL) |
PURE Output Type: | Article |
Published Date: | 2017-03-08 |
Accepted Date: | 2017-01-25 |
Submitted Date: | 2016-10-12 |
Authors: |
Gu, Junzhong
Zhang, Huiyuan Ji, Bingyang Jiang, Hui Zhao, Tao Jiang, Rongcai Zhang, Zhiren Tan, Shengjiang Ahmed, Asif ( 0000-0002-8755-8546) Gu, Yuchun ( 0000-0002-7558-0447) |
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License: Creative Commons Attribution