Luo, Binbin, Wu, Shengxi, Zhang, Zhonghao, Zou, Wengen, Shi, Shenghui, Zhao, Mingfu, Zhong, Nianbing, Liu, Yong, Zou, Xue, Wang, Lingling, Chai, Weina, Hu, Chuanmin and Zhang, Lin (2017). Human heart failure biomarker immunosensor based on excessively tilted fiber gratings. Biomedical Optics Express, 8 (1), pp. 57-67.
Abstract
A label-free immunosensor platform based on excessively tilted fiber gratings (Ex- TFGs) was developed for highly specific and fast detection of human N-terminal pro-B-type natriuretic peptide (NT-proBNP), which is considered a powerful biomarker for prognosis and risk stratification of heart failure (HF). High-purity anti-NT-proBNP monoclonal antibodies (MAbs) prepared in our laboratory were immobilized on fiber surface through the staphylococcal protein A (SPA) method for subsequent specific binding of the targeted NTproBNP. Utilizing fiber optic grating demodulation system (FOGDS), immunoassays were carried out in vitro by monitoring the resonance wavelength shift of Ex-TFG biosensor with immobilized anti-NT-proBNP MAbs. Lowest detectable concentration of ~0.5ng/mL for NTproBNP was obtained, and average sensitivity for NT-proBNP at a concentration range of 0~1.0 ng/mL was approximately 45.967 pm/(ng/mL). Several human serum samples were assessed by the proposed Ex-TFG biomarker sensor, with high specificity for NT-proBNP, indicating potential application in early diagnosing patients with acute HF symptoms.
Publication DOI: | https://doi.org/10.1364/BOE.8.000057 |
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Divisions: | College of Engineering & Physical Sciences > Aston Institute of Photonics Technology (AIPT) |
Additional Information: | © 2016 Optical Society of America |
Uncontrolled Keywords: | biological sensing and sensors,fiber optics sensors,medical optics and biotechnology,Biotechnology,Atomic and Molecular Physics, and Optics |
Publication ISSN: | 2156-7085 |
Last Modified: | 30 Sep 2024 11:03 |
Date Deposited: | 24 Jan 2017 10:00 |
Full Text Link: | |
Related URLs: |
http://www.scop ... tnerID=8YFLogxK
(Scopus URL) |
PURE Output Type: | Article |
Published Date: | 2017-01-01 |
Published Online Date: | 2016-12-05 |
Accepted Date: | 2016-11-30 |
Submitted Date: | 2016-08-01 |
Authors: |
Luo, Binbin
Wu, Shengxi Zhang, Zhonghao Zou, Wengen Shi, Shenghui Zhao, Mingfu Zhong, Nianbing Liu, Yong Zou, Xue Wang, Lingling Chai, Weina Hu, Chuanmin Zhang, Lin ( 0000-0002-1691-5577) |