Label-free oligonucleotide biosensor based on dual-peak long period fiber grating


We report the simplification and development of biofunctionalization methodology based on one-step 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)-mediated reaction. The dual-peak long period grating (dLPG) has been demonstrated its inherent ultrahigh sensitivity to refractive index (RI), achieving 50-fold improvement in RI sensitivity over a standard LPG sensor used in low RI range. With the simple and efficient immobilization of unmodified oligonucleotides on sensor surface, dLPG-based biosensor has been used to monitor the hybridization of complementary oligonucleotides showing a detectable oligonucleotide concentration of 4 nM with the advantages of label-free, real-time, and ultrahigh sensitivity.

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Divisions: College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
College of Engineering & Physical Sciences > Aston Institute of Photonics Technology (AIPT)
Additional Information: Xianfeng Chen ; Chen Liu ; Marcos R. Cardoso ; Cleber R. Mendonça ; David A. Nagel ; Anna V. Hine and Lin Zhang; "Label-free oligonucleotide biosensor based on dual-peak long period fiber grating", Proc. SPIE 9702, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI, 97020S (March 7, 2016). Copyright 2016. Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. DOI:
Event Title: SPIE Photonics West BiOS 2016
Event Type: Other
Event Location: The Moscone Center
Event Dates: 2016-02-13 - 2016-02-18
Uncontrolled Keywords: DNA,EDC-mediated reaction,label-free,long period grating,oligonucleotide,optical fiber biosensor,Atomic and Molecular Physics, and Optics,Electronic, Optical and Magnetic Materials,Biomaterials,Radiology Nuclear Medicine and imaging
ISBN: 978-1-6284-1936-8
Last Modified: 29 Jan 2024 08:01
Date Deposited: 21 Dec 2016 16:50
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
http://proceedi ... ticleid=2501733 (Publisher URL)
PURE Output Type: Conference contribution
Published Date: 2016-03-07
Accepted Date: 2016-02-01
Authors: Chen, Xianfeng
Liu, Chen
Cardoso, Marcos R.
Mendonça, Cleber R.
Nagel, David A. (ORCID Profile 0000-0002-9055-1775)
Hine, Anna V. (ORCID Profile 0000-0003-4065-831X)
Zhang, Lin (ORCID Profile 0000-0002-1691-5577)



Version: Published Version

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