Chen, Xianfeng, Liu, Chen, Cardoso, Marcos R., Mendonça, Cleber R., Nagel, David A., Hine, Anna V. and Zhang, Lin (2016). Label-free oligonucleotide biosensor based on dual-peak long period fiber grating. IN: Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI. Gannot, Israel (ed.) SPIE Proceedings . USA: SPIE.
Abstract
We report the simplification and development of biofunctionalization methodology based on one-step 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)-mediated reaction. The dual-peak long period grating (dLPG) has been demonstrated its inherent ultrahigh sensitivity to refractive index (RI), achieving 50-fold improvement in RI sensitivity over a standard LPG sensor used in low RI range. With the simple and efficient immobilization of unmodified oligonucleotides on sensor surface, dLPG-based biosensor has been used to monitor the hybridization of complementary oligonucleotides showing a detectable oligonucleotide concentration of 4 nM with the advantages of label-free, real-time, and ultrahigh sensitivity.
| Publication DOI: | https://doi.org/10.1117/12.2207869 |
|---|---|
| Divisions: | College of Health & Life Sciences > School of Biosciences College of Health & Life Sciences College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine College of Engineering & Physical Sciences > Aston Institute of Photonics Technology (AIPT) Aston University (General) |
| Additional Information: | Xianfeng Chen ; Chen Liu ; Marcos R. Cardoso ; Cleber R. Mendonça ; David A. Nagel ; Anna V. Hine and Lin Zhang; "Label-free oligonucleotide biosensor based on dual-peak long period fiber grating", Proc. SPIE 9702, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI, 97020S (March 7, 2016). Copyright 2016. Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. DOI: http://dx.doi.org/10.1117/12.2207869 |
| Event Title: | SPIE Photonics West BiOS 2016 |
| Event Type: | Other |
| Event Location: | The Moscone Center |
| Event Dates: | 2016-02-13 - 2016-02-18 |
| Uncontrolled Keywords: | DNA,EDC-mediated reaction,label-free,long period grating,oligonucleotide,optical fiber biosensor,Atomic and Molecular Physics, and Optics,Electronic, Optical and Magnetic Materials,Biomaterials,Radiology Nuclear Medicine and imaging |
| ISBN: | 978-1-6284-1936-8 |
| Last Modified: | 05 Nov 2024 17:01 |
| Date Deposited: | 21 Dec 2016 16:50 |
| Full Text Link: | |
| Related URLs: |
http://www.scop ... tnerID=8YFLogxK
(Scopus URL) http://proceedi ... ticleid=2501733 (Publisher URL) |
PURE Output Type: | Conference contribution |
| Published Date: | 2016-03-07 |
| Accepted Date: | 2016-02-01 |
| Authors: |
Chen, Xianfeng
Liu, Chen Cardoso, Marcos R. Mendonça, Cleber R. Nagel, David A. ( 
0000-0002-9055-1775)
Hine, Anna V. (
0000-0003-4065-831X)
Zhang, Lin (
0000-0002-1691-5577)
|
![[img]](https://publications.aston.ac.uk/style/images/fileicons/application_pdf.png)
CORE (COnnecting REpositories)
CORE (COnnecting REpositories)