Beyond the natural proteome:nondegenerate saturation mutagenesis - methodologies and advantages


Beyond the natural proteome, high-throughput mutagenesis offers the protein engineer an opportunity to “tweak” the wild-type activity of a protein to create a recombinant protein with required attributes. Of the various approaches available, saturation mutagenesis is one of the core techniques employed by protein engineers and in recent times, nondegenerate saturation mutagenesis is emerging as the approach of choice. This review compares the current methodologies available for conducting nondegenerate saturation mutagenesis with traditional, degenerate saturation and briefly outlines the options available for screening the resulting libraries, to discover a novel protein with the required activity and/or specificity.

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Divisions: College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
Additional Information: Proteomics in biology, part A ISBN: 978-0-1280-9742-7. © 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Uncontrolled Keywords: gene shuffling,molecular evolution,directed evolution,synthetic biology,saturation mutagenesis,non-degenerate,diversity,protein engineering,antibody engineering,Biochemistry,Molecular Biology
Publication ISSN: 1557-7988
Last Modified: 06 Mar 2024 08:12
Date Deposited: 22 Nov 2016 08:10
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://www.sci ... 3688?via%3Dihub (Publisher URL)
PURE Output Type: Article
Published Date: 2017-01-03
Accepted Date: 2016-11-07
Authors: Ferreira Amaral, M.M.
Frigotto, L.
Hine, A.V. (ORCID Profile 0000-0003-4065-831X)

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