PDNAsite:identification of DNA-binding site from protein sequence by incorporating spatial and sequence context

Abstract

Protein-DNA interactions are involved in many fundamental biological processes essential for cellular function. Most of the existing computational approaches employed only the sequence context of the target residue for its prediction. In the present study, for each target residue, we applied both the spatial context and the sequence context to construct the feature space. Subsequently, Latent Semantic Analysis (LSA) was applied to remove the redundancies in the feature space. Finally, a predictor (PDNAsite) was developed through the integration of the support vector machines (SVM) classifier and ensemble learning. Results on the PDNA-62 and the PDNA-224 datasets demonstrate that features extracted from spatial context provide more information than those from sequence context and the combination of them gives more performance gain. An analysis of the number of binding sites in the spatial context of the target site indicates that the interactions between binding sites next to each other are important for protein-DNA recognition and their binding ability. The comparison between our proposed PDNAsite method and the existing methods indicate that PDNAsite outperforms most of the existing methods and is a useful tool for DNA-binding site identification. A web-server of our predictor (http://hlt.hitsz.edu.cn:8080/PDNAsite/) is made available for free public accessible to the biological research community.

Publication DOI: https://doi.org/10.1038/srep27653
Divisions: College of Engineering & Physical Sciences > Systems analytics research institute (SARI)
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Additional Information: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Uncontrolled Keywords: General
Publication ISSN: 2045-2322
Last Modified: 27 May 2024 07:16
Date Deposited: 05 Jul 2016 10:45
Full Text Link: http://www.natu ... icles/srep27653
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2016-06-10
Accepted Date: 2016-05-18
Submitted Date: 2015-12-02
Authors: Zhou, Jiyun
Xu, Ruifeng
He, Yulan (ORCID Profile 0000-0003-3948-5845)
Lu, Qin
Wang, Hongpeng
Kong, Bing

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