The synthesis of recombinant membrane proteins in yeast for structural studies


Historically, recombinant membrane protein production has been a major challenge meaning that many fewer membrane protein structures have been published than those of soluble proteins. However, there has been a recent, almost exponential increase in the number of membrane protein structures being deposited in the Protein Data Bank. This suggests that empirical methods are now available that can ensure the required protein supply for these difficult targets. This review focuses on methods that are available for protein production in yeast, which is an important source of recombinant eukaryotic membrane proteins. We provide an overview of approaches to optimize the expression plasmid, host cell and culture conditions, as well as the extraction and purification of functional protein for crystallization trials in preparation for structural studies.

Publication DOI:
Divisions: College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
Additional Information: Funding: BBSRC (grants BB/I019960/1, BB/K013319/1, BB/L502194/1 and BB/L015846/1; and the Innovative Medicines Joint Undertaking (grant 115583 to the ND4BB ENABLE Consortium)
Uncontrolled Keywords: membrane proteins,yeast,recombinant antigen,expression plasmid vector,promoter,SMALP,cholesterol,Molecular Biology,Biochemistry, Genetics and Molecular Biology(all)
Publication ISSN: 1095-9130
Last Modified: 28 Feb 2024 08:12
Date Deposited: 07 Dec 2015 09:40
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2016-02-15
Published Online Date: 2015-09-30
Accepted Date: 2015-09-29
Submitted Date: 2015-07-25
Authors: Routledge, Sarah
Mikaliunaite, Lina
Patel, Anjana
Clare, Michelle
Cartwright, Stephanie P.
Bawa, Zharain
Wilks, Martin D.B.
Low, Floren
Hardy, David
Rothnie, Alice J. (ORCID Profile 0000-0002-4259-7015)
Bill, Roslyn M. (ORCID Profile 0000-0003-1331-0852)



Version: Published Version

License: Creative Commons Attribution


Version: Accepted Version

Access Restriction: Restricted to Repository staff only

Export / Share Citation


Additional statistics for this record