EDC-mediated oligonucleotide immobilization on a long period grating optical biosensor


We present the development and simplification of label-free fiber optic biosensors based on immobilization of oligonucleotides on dual-peak long period gratings (dLPGs). This improvement is the result of a simplification of biofunctionalization methodology. A one-step 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-mediated reaction has been developed for the straightforward immobilization of unmodified oligonucleotides on the glass fiber surface along the grating region, leading to covalent attachment of a 5´-phosphorylated probe oligonucleotide to the amino-derivatized fiber grating surface. Immobilization is achieved via a 5´phosphate-specific linkage, leaving the remainder of the oligonucleotide accessible for binding reactions. The dLPG has been tested in different external media to demonstrate its inherent ultrahigh sensitivity to the surrounding-medium refractive index (RI) achieving 50- fold improvement in RI sensitivity over the previously-published LPG sensor in media with RI’s relevant to biological assays. After functionalization, the dLPG biosensor was used to monitor the hybridization of complementary oligonucleotides showing a detectable oligonucleotide concentration of 4 nM. The proposed one-step EDC reaction approach can be further extended to develop fiber optic biosensors for disease analysis and medical diagnosis with the advances of label-free, real-time, multiplex, high sensitivity and specificity.

Publication DOI: https://doi.org/10.4172/2155-6210.1000173
Divisions: College of Health & Life Sciences > Aston Pharmacy School
College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
College of Engineering & Physical Sciences > Aston Institute of Photonics Technology (AIPT)
Additional Information: © 2015 Chen X, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: FP7 PIRSES-2013-612267 and the Ser Cymru NRN.
Uncontrolled Keywords: optical fiber biosensor,long period grating,label-free,oligonucleotide,DNA,EDC-mediated reaction
Publication ISSN: 2155-6210
Last Modified: 22 May 2024 07:12
Date Deposited: 14 Oct 2015 14:05
Full Text Link: http://www.omic ... 210-1000173.pdf
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PURE Output Type: Article
Published Date: 2015-06-28
Authors: Chen, Xianfeng
Liu, Chen
Hughes, Marcus D.
Nagel, David A. (ORCID Profile 0000-0002-9055-1775)
Hine, Anna V. (ORCID Profile 0000-0003-4065-831X)
Zhang, Lin (ORCID Profile 0000-0002-1691-5577)



Version: Published Version

License: Creative Commons Attribution

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