Traikov, Sofia, Stange, Christoph, Wassmer, Thomas, Paul-Gilloteaux, Perrine, Salamero, Jean, Raposo, Graça and Hoflack, Bernard (2014). Septin6 and Septin7 GTP binding proteins regulate AP-3- and ESCRT-dependent multivesicular body biogenesis. PLoS ONE, 9 (11),
Abstract
Septins (SEPTs) form a family of GTP-binding proteins implicated in cytoskeleton and membrane organization, cell division and host/pathogen interactions. The precise function of many family members remains elusive. We show that SEPT6 and SEPT7 complexes bound to F-actin regulate protein sorting during multivesicular body (MVB) biogenesis. These complexes bind AP-3, an adapter complex sorting cargos destined to remain in outer membranes of maturing endosomes, modulate AP-3 membrane interactions and the motility of AP-3-positive endosomes. These SEPT-AP interactions also influence the membrane interaction of ESCRT (endosomal-sorting complex required for transport)-I, which selects ubiquitinated cargos for degradation inside MVBs. Whereas our findings demonstrate that SEPT6 and SEPT7 function in the spatial, temporal organization of AP-3- and ESCRT-coated membrane domains, they uncover an unsuspected coordination of these sorting machineries during MVB biogenesis. This requires the E3 ubiquitin ligase LRSAM1, an AP-3 interactor regulating ESCRT-I sorting activity and whose mutations are linked with Charcot-Marie-Tooth neuropathies.
Publication DOI: | https://doi.org/10.1371/journal.pone.0109372 |
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Divisions: | College of Health & Life Sciences > School of Biosciences College of Health & Life Sciences College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine |
Additional Information: | Copyright: © 2014 Traikov et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
Uncontrolled Keywords: | General Agricultural and Biological Sciences,General Biochemistry,Genetics and Molecular Biology,General Medicine |
Publication ISSN: | 1932-6203 |
Last Modified: | 05 Nov 2024 08:07 |
Date Deposited: | 23 Jun 2015 12:25 |
Full Text Link: |
http://journals ... al.pone.0109372 |
Related URLs: |
http://www.scop ... tnerID=8YFLogxK
(Scopus URL) |
PURE Output Type: | Article |
Published Date: | 2014-11-07 |
Authors: |
Traikov, Sofia
Stange, Christoph Wassmer, Thomas Paul-Gilloteaux, Perrine Salamero, Jean Raposo, Graça Hoflack, Bernard |