Hijacked then lost in translation:the plight of the recombinant host cell in membrane protein structural biology projects


Membrane protein structural biology is critically dependent upon the supply of high-quality protein. Over the last few years, the value of crystallising biochemically characterised, recombinant targets that incorporate stabilising mutations has been established. Nonetheless, obtaining sufficient yields of many recombinant membrane proteins is still a major challenge. Solutions are now emerging based on an improved understanding of recombinant host cells; as a 'cell factory' each cell is tasked with managing limited resources to simultaneously balance its own growth demands with those imposed by an expression plasmid. This review examines emerging insights into the role of translation and protein folding in defining high-yielding recombinant membrane protein production in a range of host cells.

Publication DOI: https://doi.org/10.1016/j.sbi.2015.04.003
Divisions: College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences
College of Health & Life Sciences > School of Biosciences > Cellular and Molecular Biomedicine
Additional Information: Funding: Biotechnology and Biological Sciences Research Council (BBSRC; grant BB/I019960/1) and the Innovative Medicines Joint Undertaking under Grant Agreement no. 115583 to the ND4BB ENABLE Consortium. BBSRC (grant BB/I010351/1) and from the Leverhulme Trust (grant RPG-2014-032).
Uncontrolled Keywords: Molecular Biology,Structural Biology
Publication ISSN: 1879-033X
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2015-06
Published Online Date: 2015-06-01
Authors: Bill, Roslyn M. (ORCID Profile 0000-0003-1331-0852)
von der Haar, Tobias



Version: Published Version

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