Salama, Rima, Sattayasai, Jintana, Gande, Arun Kumar, Sattayasai, Nison, Davis, Mike and Lattmann, Eric (2012). Identification and evaluation of agents isolated from traditionally used herbs against Ophiophagus hannah venom. Drug Discoveries and Therapeutics, 6 (1), pp. 18-23.
Abstract
The aim of this study was firstly to identify active molecules in herbs, that are traditionally used for the treatment of snake bite, such as Curcuma antinaia, Curcuma contravenenum, Andrographis paniculata, and Tanacetum parthenium; secondly to test similar structurally related molecules and finally to prepare and evaluate an efficient formulation against Ophiophagus hannah venom intoxification. Three labdane based compounds, including labdane dialdehyde, labdane lactone, and labdane trialdehyde and two lactones including 14-deoxy-11,12-didehydroandrographolide and parthenolide were isolated by column chromatography and characterised. Using the isolated rat phrenic nerve-hemidiaphragm preparation, the antagonistic effect of crude extracts, isolated compounds and prepared formulations were measured in vitro on the inhibition of the neuromuscular transmission. Inhibition on muscle contraction, produced by the 5 μg/mL venom, was reversed by test agents in organ bath preparations. A labdane trialdehyde, isolated from C. contravenenum, was identified as the best antagonising agent in the low micromolar range. Tests on formulations of the most potent C. contravenenum extract showed, that the suppository with witepsol H15 was an effective medicine against O. hannah venom. This study elucidated the active compounds, accounting for the antivenin activity of traditionally used herbs and suggested the most suitable formulation, which may help to develop potent medicines for the treatment of snake bite in the future.
Publication DOI: | https://doi.org/10.5582/ddt.2012.v6.1.18 |
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Divisions: | College of Health & Life Sciences > Aston Pharmacy School College of Health & Life Sciences Aston University (General) |
Uncontrolled Keywords: | antivenin activity,rat nerve-hemidiapragm,labdane diadehyde,labdane triadehyde,parthenolide,desoxy-andrographolide,General Medicine |
Publication ISSN: | 1881-784X |
Last Modified: | 05 Nov 2024 08:08 |
Date Deposited: | 01 Jun 2015 08:55 |
Full Text Link: | |
Related URLs: |
http://www.scop ... tnerID=8YFLogxK
(Scopus URL) http://www.ddtj ... ract.php?id=504 (Publisher URL) |
PURE Output Type: | Article |
Published Date: | 2012-02-01 |
Authors: |
Salama, Rima
Sattayasai, Jintana Gande, Arun Kumar Sattayasai, Nison Davis, Mike Lattmann, Eric |