Selective AKR1C3 inhibitors do not recapitulate the anti-leukaemic activities of the pan-AKR1C inhibitor medroxyprogesterone acetate

Abstract

Background: We and others have identified the aldo-keto reductase AKR1C3 as a potential drug target in prostate cancer, breast cancer and leukaemia. As a consequence, significant effort is being invested in the development of AKR1C3-selective inhibitors. Methods: We report the screening of an in-house drug library to identify known drugs that selectively inhibit AKR1C3 over the closely related isoforms AKR1C1, 1C2 and 1C4. This screen initially identified tetracycline as a potential AKR1C3-selective inhibitor. However, mass spectrometry and nuclear magnetic resonance studies identified that the active agent was a novel breakdown product (4-methyl(de-dimethylamine)-tetracycline (4-MDDT)). Results: We demonstrate that, although 4-MDDT enters AML cells and inhibits their AKR1C3 activity, it does not recapitulate the anti-leukaemic actions of the pan-AKR1C inhibitor medroxyprogesterone acetate (MPA). Screens of the NCI diversity set and an independently curated small-molecule library identified several additional AKR1C3-selective inhibitors, none of which had the expected anti-leukaemic activity. However, a pan AKR1C, also identified in the NCI diversity set faithfully recapitulated the actions of MPA. Conclusions: In summary, we have identified a novel tetracycline-derived product that provides an excellent lead structure with proven drug-like qualities for the development of AKR1C3 inhibitors. However, our findings suggest that, at least in leukaemia, selective inhibition of AKR1C3 is insufficient to elicit an anticancer effect and that multiple AKR1C inhibition may be required.

Publication DOI: https://doi.org/10.1038/bjc.2014.83
Divisions: College of Health & Life Sciences > School of Biosciences
Additional Information: Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0) Funding: Leukaemia and Lymphoma Research (LLR)
Uncontrolled Keywords: aldo-keto reductase 1C3,inhibitors,leukaemia,Cancer Research,Oncology
Publication ISSN: 1532-1827
Last Modified: 22 Mar 2024 08:12
Date Deposited: 07 May 2015 08:15
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
http://www.natu ... bjc201483a.html (Publisher URL)
PURE Output Type: Article
Published Date: 2014-03-18
Published Online Date: 2014-02-25
Authors: Khanim, F.
Davies, N.
Veliça, P.
Hayden, R.
Ride, J.
Pararasa, C.
Chong, M.G.
Gunther, U.
Veerapen, N.
Winn, P.
Farmer, R.
Trivier, E.
Rigoreau, L.
Drayson, M.
Bunce, C.

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