Differential effects of chlorinated and oxidized phospholipids in vascular tissue:implications for neointima formation


The presence of inflammatory cells and MPO (myeloperoxidase) in the arterial wall after vascular injury could increase neointima formation by modification of phospholipids. The present study investigates how these phospholipids, in particular oxidized and chlorinated species, are altered within injured vessels and how they affect VSMC (vascular smooth muscle cell) remodelling processes. Vascular injury was induced in C57BL/6 mice and high fat-fed ApoE-/- (apolipoprotein E) mice by wire denudation and ligation of the left carotid artery (LCA). Neointimal and medial composition was assessed using immunohistochemistry and ESI-MS. Primary rabbit aortic SMCs (smooth muscle cells) were utilized to examine the effects of modified lipids on VSMC proliferation, viability and migration at a cellular level. Neointimal area, measured as intima-to-media ratio, was significantly larger in wire-injured ApoE-/- mice (3.62±0.49 compared with 0.83±0.25 in C57BL/6 mice, n=3) and there was increased oxidized low-density lipoprotein (oxLDL) infiltration and elevated plasma MPO levels. Relative increases in lysophosphatidylcholines and unsaturated phosphatidylcholines (PCs) were also observed in wire-injured ApoE-/- carotid arteries. Chlorinated lipids had no effect on VSMC proliferation, viability or migration whereas chronic incubation with oxidized phospholipids stimulated proliferation in the presence of fetal calf serum [154.8±14.2% of viable cells at 1 μM PGPC (1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphocholine) compared with control, n=6]. In conclusion, ApoE-/- mice with an inflammatory phenotype develop more neointima in wire-injured arteries and accumulation of oxidized lipids in the vessel wall may propagate this effect.

Publication DOI: https://doi.org/10.1042/CS20140578
Divisions: College of Health & Life Sciences > School of Biosciences
College of Health & Life Sciences > Chronic and Communicable Conditions
College of Health & Life Sciences
Additional Information: This research was originally published in Clinical Science.Greig, F. H., Hutchison, L., Spickett, C. M., & Kennedy, S. (2015). Differential effects of chlorinated and oxidized phospholipids in vascular tissue: implications for neointima formation. Clinical Science, 128(9), 579-592; Published as Immediate Publication © the Biochemical Society
Uncontrolled Keywords: General Medicine
Publication ISSN: 1470-8736
Last Modified: 27 Jun 2024 08:26
Date Deposited: 26 Jan 2015 11:05
Full Text Link: http://www.clin ... /CS20140578.htm
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
PURE Output Type: Article
Published Date: 2015-05-01
Authors: Greig, Fiona H.
Hutchison, Lisa
Spickett, Corinne M. (ORCID Profile 0000-0003-4054-9279)
Kennedy, Simon



Version: Accepted Version

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